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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology

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Author(s):
Lorenzi, Adriana Sturion [1, 2] ; Chia, Mathias Ahii [2, 3] ; Cardoso Lopes, Fabyano Alvares [1, 4] ; Silva, Genivaldo Gueiros Z. [1] ; Edwards, Robert A. [5, 1] ; Bittencourt-Oliveira, Maria do Carmo [2]
Total Authors: 6
Affiliation:
[1] San Diego State Univ, Computat Sci Res Ctr, San Diego, CA 92182 - USA
[2] Univ Sao Paulo, Luiz Queiroz Coll Agr, Dept Biol Sci, Lab Cyanobacteria, Piracicaba, SP - Brazil
[3] SUNY Stony Brook, Sch Marine & Atmospher Sci, Southampton Campus, Stony Brook, NY 11794 - USA
[4] Univ Brasilia UNB, Dept Cell Biol, Lab Enzymol, Brasilia, DF - Brazil
[5] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF MICROBIOLOGY; v. 57, n. 6, p. 450-460, JUN 2019.
Web of Science Citations: 0
Abstract

Next-generation DNA sequencing technology was applied to generate molecular data from semiarid reservoirs during well-defined seasons. Target sequences of 16S-23S rRNA ITS and cpcBA-IGS were used to reveal the taxonomic groups of cyanobacteria present in the samples, and genes coding for cyanotoxins such as microcystins (mcyE), saxitoxins (sxtA), and cylindrospermopsins (cyrJ) were investigated. The presence of saxitoxins in the environmental samples was evaluated using ELISA kit. Taxonomic analyses of high-throughput DNA sequencing data showed the dominance of the genus Microcystis in Mundau reservoir. Furthermore, it was the most abundant genus in the dry season in Ingazeira reservoir. In the rainy season, 16S-23S rRNA ITS analysis revealed that Cylindrospermopsis raciborskii comprised 46.8% of the cyanobacterial community in Ingazeira reservoir, while the cpcBAIGS region revealed that C. raciborskii (31.8%) was the most abundant taxon followed by Sphaerospermopsis aphanizomenoides (17.3%) and Planktothrix zahidii (16.6%). Despite the presence of other potential toxin-producing genera, the detected sxtA gene belonged to C. raciborskii, while the mcyE gene belonged to Microcystis in both reservoirs. The detected mcyE gene had good correlation with MC content, while the amplification of the sxtA gene was related to the presence of STX. The cyrJ gene was not detected in these samples. Using DNA analyses, our results showed that the cyanobacterial composition of Mundau reservoir was similar in successive dry seasons, and it varied between seasons in Ingazeira reservoir. In addition, our data suggest that some biases of analysis influenced the cyanobacterial communities seen in the NGS output of Ingazeira reservoir. (AU)

FAPESP's process: 13/11306-3 - The Effects of anatoxin-a and cylindrospermopsin at different nitrogen concentrations and photon irradiances on the physiology of Microcystis aeruginosa (Cyanobacteria) and Scenedesmus acuminatus (Chlolrophyta)
Grantee:Mathias Ahii Chia
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/01913-2 - Comparative Metagenomics of cyanobacterial blooms in freshwater reservoirs from Pernambuco State
Grantee:Adriana Sturion Lorenzi
Support Opportunities: Scholarships in Brazil - Young Researchers
FAPESP's process: 13/15296-2 - Comparative metagenomics of cyanobacterial blooms in freshwater reservoirs from Pernambuco State
Grantee:Adriana Sturion Lorenzi
Support Opportunities: Research Grants - Young Investigators Grants