N-Acetylcysteine reverses silver nanoparticle intoxication in rats

Autor(es)

Padilha Mendonca, Monique Culturato ^[1] ; Ferreira, Luiz Bandeira ^[1] ; Rizoli, Cintia ^[1] ; Batista, Angela Giovana ^[2] ; Marostica Junior, Mario Roberto ^[2] ; da Silva, Emanueli do Nascimento ^[3] ; Cadore, Solange ^[3] ; Duran, Nelson ^{[3, 4]} ; da Cruz-Hofling, Maria Alice ^[1] ; de Jesus, Marcelo Bispo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, R Monteiro Lobato, 255 Cidade Univ, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Food & Nutr, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[4] Fed Univ ABC UFABC, Nanomed Units, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Nanotoxicology; v. 13, n. 3, p. 326-338, MAR 16 2019.
• Citações Web of Science: 1

Resumo

The increasing use of silver nanoparticles (AgNPs) in consumer products raises the risk of human toxicity. Currently, there are no therapeutic options or established treatment protocols in cases of AgNPs intoxication. We demonstrated previously that thiol antioxidants compounds can reverse the cytotoxicity induced by AgNPs in Huh-7 hepatocarcinoma cells. Here, we investigated the use of N-acetylcysteine (NAC) against the systemic toxic effects of AgNPs (79.3 nm) in rats. Biochemical, histopathological, hematological, and oxidative parameters showed that a single intravenous injection of AgNPs (5 mg/kg b.w.) induced deleterious effects such as hepatotoxicity, potentially as a result of AgNPs accumulation in the liver. Treatment with a single intraperitoneal injection of NAC (1 g/kg b.w.) one hour after AgNPs exposure significantly attenuated all toxic effects evaluated and altered the bioaccumulation and release patterns of AgNPs in rats. The findings show that NAC may be a promising candidate for clinical management of AgNPs intoxication. (AU)

• Processo FAPESP: 14/03002-7 - Internalização e tráfego intracelular de nanopartículas: atividade biológica e perfil nanotoxicológico
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/03765-6 - Determinação in vitro e in vivo do perfil toxicológico de formulações utilizando nanotecnologia
• Beneficiário: Monique Culturato Padilha Mendonça
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/06134-4 - EMU concedido no processo 2014/03002-7: leitor multi-modo de imageamento celular (cell imaging multi-mode reader)
• Beneficiário: Marcelo Bispo de Jesus
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários