N-Acetylcysteine reverses silver nanoparticle intoxication in rats

Author(s)

Padilha Mendonca, Monique Culturato ^[1] ; Ferreira, Luiz Bandeira ^[1] ; Rizoli, Cintia ^[1] ; Batista, Angela Giovana ^[2] ; Marostica Junior, Mario Roberto ^[2] ; da Silva, Emanueli do Nascimento ^[3] ; Cadore, Solange ^[3] ; Duran, Nelson ^{[3, 4]} ; da Cruz-Hofling, Maria Alice ^[1] ; de Jesus, Marcelo Bispo ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, R Monteiro Lobato, 255 Cidade Univ, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Food & Nutr, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[4] Fed Univ ABC UFABC, Nanomed Units, Sao Paulo - Brazil

• Document type: Journal article
• Source: Nanotoxicology; v. 13, n. 3, p. 326-338, MAR 16 2019.
• Web of Science Citations: 1

Abstract

The increasing use of silver nanoparticles (AgNPs) in consumer products raises the risk of human toxicity. Currently, there are no therapeutic options or established treatment protocols in cases of AgNPs intoxication. We demonstrated previously that thiol antioxidants compounds can reverse the cytotoxicity induced by AgNPs in Huh-7 hepatocarcinoma cells. Here, we investigated the use of N-acetylcysteine (NAC) against the systemic toxic effects of AgNPs (79.3 nm) in rats. Biochemical, histopathological, hematological, and oxidative parameters showed that a single intravenous injection of AgNPs (5 mg/kg b.w.) induced deleterious effects such as hepatotoxicity, potentially as a result of AgNPs accumulation in the liver. Treatment with a single intraperitoneal injection of NAC (1 g/kg b.w.) one hour after AgNPs exposure significantly attenuated all toxic effects evaluated and altered the bioaccumulation and release patterns of AgNPs in rats. The findings show that NAC may be a promising candidate for clinical management of AgNPs intoxication. (AU)

• FAPESP's process: 14/03002-7 - Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile
• Grantee: Marcelo Bispo de Jesus
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/03765-6 - In vitro and in vivo determination of toxicological profile of nanoformulations
• Grantee: Monique Culturato Padilha Mendonça
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/06134-4 - Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader
• Grantee: Marcelo Bispo de Jesus
• Support Opportunities: Multi-user Equipment Program