Extract obtaining and synthesis of silver nanoparticles from Punica granatum (pomegranate): Physico-chemical characterization, determination of antimicrobial activity and potential healing

• Grant number: 16/04230-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2016
• End date: September 21, 2017
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Debora Barros Barbosa
• Grantee: Renan Aparecido Fernandes
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study is to produce extracts from the bark of Punica granatum (pomegranate) and synthesize silver nanoparticles from these extracts (synthesis green), characterize them, evaluate the minimum inhibitory concentration aigainst Candida albicans SC5314 and Staphylococcus aureus ATCC 25923 and its effect on wound repair reproduced in mice and infected with the same microorganism. The extracts will be obtained by alcoholic route and will be characterized physico-chemically as pharmacopoeic analysis and high-performance liquid chromatography (HPLC). They will be used to reduce silver nitrate in silver nanoparticles, and the presence of silver nanoparticles will be confirmed by TEM, SEM and UV-Vis spectroscopy. The minimum inhibitory concentration will be determined for all compounds (extracts silver nanoparticles). After the identification of ideal concentrations of the compound a semisolid formulation will be developed and tested in would reproduced in mice verifying its anti-inflammatory potential and also its antimicrobial efficacy against mixed biofilm of C. albicans and S. aureus by counting colony forming units (UFC) in model in vivo. Data will be subjected to normality test and then appropriate statistical analysis will be applied for each outcome with the 5% significance level.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES SCAPPATICCI, RENAN APARECIDO; BERRETTA, ANDRESA APARECIDA; TORRES, ELINA CASSIA; MOREIRA BUSZINSKI, ANDREI FELIPE; FERNANDES, GABRIELA LOPES; DOS REIS, THAILA FERNANDA; DE SOUZA-NETO, FRANCISCO NUNES; GORUP, LUIZ FERNANDO; DE CAMARGO, EMERSON RODRIGUES; BARBOSA, DEBORA BARROS. Green and Chemical Silver Nanoparticles and Pomegranate Formulations to Heal Infected Wounds in Diabetic Rats. ANTIBIOTICS-BASEL, v. 10, n. 11, NOV 2021. (16/04230-9)

FERNANDES, RENAN APARECIDO; BERRETTA, ANDRESA APARECIDA; TORRES, ELINA CASSIA; MOREIRA BUSZINSKI, ANDREI FELIPE; FERNANDES, GABRIELA LOPES; MENDES-GOUVEA, CARLA CORREA; DE SOUZA-NETO, FRANCISCO NUNES; GORUP, LUIZ FERNANDO; DE CAMARGO, EMERSON RODRIGUES; BARBOSA, DEBORA BARROS. Antimicrobial Potential and Cytotoxicity of Silver Nanoparticles Phytosynthesized by Pomegranate Peel Extract. ANTIBIOTICS-BASEL, v. 7, n. 3, SEP 2018. (16/04230-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERNANDES, Renan Aparecido. Fitossíntese de nanopartículas de prata a partir de extrato de cascas de romã e desenvolvimento de formulação para tratamento de feridas: avaliação antimicrobiana, citotóxica e potencial cicatrizante em um modelo in vivo. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.