Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader

• Grant number: 15/06134-4
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2015
• End date: October 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Marcelo Bispo de Jesus
• Grantee: Marcelo Bispo de Jesus
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/03002-7 - Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile, AP.JP
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Caracterização - Leitores de fluorescência (imageamento, placas); Processos Biológicos - Caracterização - Leitores de placas
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The multi-modal imaging system (Cytation " 5) is a configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate reading to provide phenotypic cellular information and well-based quantitative data. This single instrument platform can process workflows that would traditionally require multiple instruments and software interfaces, and is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular imaging in fluorescence, brightfield, color brightfield and phase contrast. Live cell imaging and multi-mode assays are optimized with incubation to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors (modules which can be acquired separately). Image capture, data collection and powerful image and data analysis are managed with Gen5" software, specifically designed for uncomplicated processing of even the most complex assays, such as cell proliferation and viability, evaluation of oxidative stress levels, quantification of proteins and nucleic acids, cell death apoptosis/necrosis gene expression reporter gene, signal transduction, transfection efficiency, cell cycle, protein translocation to the nucleus, among others. The analysis of these processes in real time allows evaluating the interaction of cells for many different purposes (cell-cell interaction studies, cell-bacteria, active cells, cell-nanoparticles) and is of great value and interest of researchers of the Institute of Biology of UNICAMP. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; et al. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, APR 16 2019. (17/01451-7, 12/10653-9, 16/23142-3, 13/06810-4, 14/03002-7, 15/01507-7, 18/01753-6, 15/06134-4, 17/25679-7, 17/06577-9, 18/02208-1, 17/26366-2, 17/16280-3)

GARCIA-FOSSA, FERNANDA; GAAL, VLADIMIR; DE JESUS, MARCELO BISPO. PyScratch: An ease of use tool for analysis of scratch assays. COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE, v. 193, SEP 2020. (14/03002-7, 15/06134-4)

BARRETO, NATALIA; CABALLERO, MARCUS; BONFANTI, AMANDA PIRES; PINHEIRO DE MATO, FELIPE CEZAR; MUNHOZ, JAQUELINE; DA ROCHA-E-SILVA, THOMAZ A. A.; SUTTI, RAFAEL; VITORINO-ARAUJO, JOAO LUIZ; VERINAUD, LIANA; RAPOSO, CATARINA. Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na+/K+-ATPase beta 2: potential molecular entities to treat invasive brain cancer. CANCER CELL INTERNATIONAL, v. 20, n. 1, DEC 17 2020. (15/04194-0, 15/06134-4, 17/05402-0, 17/16196-2, 17/24331-7, 19/10003-3, 18/23559-7)

JORGE, G. PEREZ; MODOLO, D. G.; JAIMES-FLOREZ, Y. P.; FAVARO, W. J.; DE JESUS, M. B.; BROCCHI, M.. p53 gene delivery via a recombinant Salmonella enterica Typhimurium leads to human bladder carcinoma cell death in vitro. Letters in Applied Microbiology, v. 75, n. 4, p. 11-pg., 2022-07-02. (17/10051-2, 15/06134-4)

BANDEIRA FERREIRA, LUIZ ALBERTO; GARCIA-FOSSA, FERNANDA; RADAIC, ALLAN; DURAN, NELSON; FAVARO, WAGNER JOSE; DE JESUS, MARCELO BISPO. Biogenic silver nanoparticles: In vitro and in vivo antitumor activity in bladder cancer. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 151, p. 162-170, JUN 2020. (15/06134-4, 14/03002-7)

PADILHA MENDONCA, MONIQUE CULTURATO; FERREIRA, LUIZ BANDEIRA; RIZOLI, CINTIA; BATISTA, ANGELA GIOVANA; MAROSTICA JUNIOR, MARIO ROBERTO; DA SILVA, EMANUELI DO NASCIMENTO; CADORE, SOLANGE; DURAN, NELSON; DA CRUZ-HOFLING, MARIA ALICE; DE JESUS, MARCELO BISPO. N-Acetylcysteine reverses silver nanoparticle intoxication in rats. Nanotoxicology, v. 13, n. 3, p. 326-338, MAR 16 2019. (14/03002-7, 16/03765-6, 15/06134-4)

FERREIRA, LUIZ A. B.; DOS REIS, SAMARA BONESSO; DA SILVA, EMANUELI DO NASCIMENTO; CADORE, SOLANGE; BERNARDES, JULIANA DA SILVA; DURAN, NELSON; DE JESUS, MARCELO B.. Thiol-antioxidants interfere with assessing silver nanoparticle cytotoxicity. Nanomedicine-Nanotechnology Biology and Medicine, v. 24, FEB 2020. (15/06134-4, 14/03002-7)

DOS REIS, SAMARA BONESSO; SILVA, JULIANA DE OLIVEIRA; GARCIA-FOSSA, FERNANDA; LEITE, ELAINE AMARAL; MALACHIAS, ANGELO; POUND-LANA, GWENAELLE; FURTADO MOSQUEIRA, VANESSA CARLA; OLIVEIRA, MONICA CRISTINA; BRANCO DE BARROS, ANDRE LUIS; DE JESUS, MARCELO BISPO. Mechanistic insights into the intracellular release of doxorubicin from pH-sensitive liposomes. BIOMEDICINE & PHARMACOTHERAPY, v. 134, FEB 2021. (14/03002-7, 15/06134-4)

RADAIC, A.; DE JESUS, M. B.. Solid lipid nanoparticles release DNA upon endosomal acidification in human embryonic kidney cells. Nanotechnology, v. 29, n. 31, AUG 3 2018. (14/03002-7, 15/06134-4)

BONFANTI, AMANDA PIRES; BARRETO, NATALIA; MUNHOZ, JAQUELINE; CABALLERO, MARCUS; CORDEIRO, GABRIEL; ROCHA-E-SILVA, THOMAZ; SUTTI, RAFAEL; MOURA, FERNANDA; BRUNETTO, SERGIO; RAMOS, CELSO DARIO; et al. Spider venom administration impairs glioblastoma growth and modulates immune response in a non-clinical model. SCIENTIFIC REPORTS, v. 10, n. 1, APR 3 2020. (18/03051-9, 16/15827-6, 17/05402-0, 15/06134-4, 17/16196-2, 15/04194-0, 18/23559-7)

BANDEIRA FERREIRA, LUIZ ALBERTO; GARCIA-FOSSA, FERNANDA; RADAIC, ALLAN; DURAN, NELSON; FAVARO, WAGNER JOSE; DE JESUS, MARCELO BISPO. Biogenic silver nanoparticles: In vitro and in vivo antitumor activity in bladder cancer. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 151, p. 9-pg., 2020-06-01. (15/06134-4, 14/03002-7)

PONTES, PAULA VIRGINIA DE ALMEIDA; CZAIKOSKI, ALINE; ALMEIDA, NAARA APARECIDA; FRAGA, SARA; ROCHA, LILIANA DE OLIVEIRA; CUNHA, ROSIANE LOPES; MAXIMO, GUILHERME JOSE; BATISTA, EDUARDO AUGUSTO CALDAS. Extraction optimization, biological activities, and application in O/W emulsion of deep eutectic solvents-based phenolic extracts from olive pomace. Food Research International, v. 161, p. 14-pg., 2022-08-11. (17/04811-4, 14/21252-0, 16/08566-1, 15/06134-4)

CZAIKOSKI, ALINE; GOMES, ANDRESA; KAUFMANN, KARINE CRISTINE; LISZBINSKI, RAQUEL BESTER; DE JESUS, MARCELO BISPO; DA CUNHA, ROSIANE LOPES. Lignin derivatives stabilizing oil-in-water emulsions: Technological aspects, interfacial rheology and cytotoxicity. INDUSTRIAL CROPS AND PRODUCTS, v. 154, OCT 15 2020. (15/06134-4)

MUNHOZ, JAQUELINE; PERON, GABRIELA; BONFANTI, AMANDA PIRES; OLIVEIRA, JANINE; DA ROCHA-E-SILVA, THOMAZ A. A.; SUTTI, RAFAEL; THOME, RODOLFO; BOMBEIRO, ANDRE LUIS; BARRETO, NATALIA; CHALBATANI, GHANBAR MAHMOODI; et al. Components from spider venom activate macrophages against glioblastoma cells: new potential adjuvants for anticancer immunotherapy. JOURNAL OF BIOCHEMISTRY, v. 170, n. 1, p. 51-68, JUL 2021. (15/04194-0, 15/06134-4, 18/23559-7, 18/03051-9, 19/10003-3)