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Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader

Grant number: 15/06134-4
Support type:Multi-user Equipment Program
Duration: November 01, 2015 - October 31, 2017
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Marcelo Bispo de Jesus
Grantee:Marcelo Bispo de Jesus
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:14/03002-7 - Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile, AP.JP
As informações de acesso ao Equipamento Multiusuário são de responsabilidade do Pesquisador responsável
EMU web page: Página do Equipamento Multiusuário não informada
Tipo de equipamento:Processos Biológicos - Caracterização - Leitores de fluorescência (imageamento, placas)
Processos Biológicos - Caracterização - Leitores de placas
Fabricante: Fabricante não informado
Modelo: Modelo não informado

Abstract

The multi-modal imaging system (Cytation " 5) is a configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate reading to provide phenotypic cellular information and well-based quantitative data. This single instrument platform can process workflows that would traditionally require multiple instruments and software interfaces, and is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular imaging in fluorescence, brightfield, color brightfield and phase contrast. Live cell imaging and multi-mode assays are optimized with incubation to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors (modules which can be acquired separately). Image capture, data collection and powerful image and data analysis are managed with Gen5" software, specifically designed for uncomplicated processing of even the most complex assays, such as cell proliferation and viability, evaluation of oxidative stress levels, quantification of proteins and nucleic acids, cell death apoptosis/necrosis gene expression reporter gene, signal transduction, transfection efficiency, cell cycle, protein translocation to the nucleus, among others. The analysis of these processes in real time allows evaluating the interaction of cells for many different purposes (cell-cell interaction studies, cell-bacteria, active cells, cell-nanoparticles) and is of great value and interest of researchers of the Institute of Biology of UNICAMP. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; MARTINS, FLAVIANO DOS SANTOS; SATO GUIMA, SUZANA EIKO; THOMAS, ANDREW MALTEZ; SETUBAL, JOAO CARLOS; MAGALHAES, YULI THAMIRES; FORTI, FABIO LUIS; CANDREVA, THAMIRIS; RODRIGUES, HOSANA GOMES; DE JESUS, MARCELO BISPO; CONSONNI, SILVIO ROBERTO; FARIAS, ALESSANDRO DOS SANTOS; VARGA-WEISZ, PATRICK; RAMIREZ VINOLO, MARCO AURELIO. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, APR 16 2019. Web of Science Citations: 3.
PADILHA MENDONCA, MONIQUE CULTURATO; FERREIRA, LUIZ BANDEIRA; RIZOLI, CINTIA; BATISTA, ANGELA GIOVANA; MAROSTICA JUNIOR, MARIO ROBERTO; DA SILVA, EMANUELI DO NASCIMENTO; CADORE, SOLANGE; DURAN, NELSON; DA CRUZ-HOFLING, MARIA ALICE; DE JESUS, MARCELO BISPO. N-Acetylcysteine reverses silver nanoparticle intoxication in rats. Nanotoxicology, v. 13, n. 3, p. 326-338, MAR 16 2019. Web of Science Citations: 1.
RADAIC, A.; DE JESUS, M. B. Solid lipid nanoparticles release DNA upon endosomal acidification in human embryonic kidney cells. Nanotechnology, v. 29, n. 31 AUG 3 2018. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.