Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism

Full text
Author(s):
Show less -
Fachi, Jose Luis [1] ; Felipe, Jaqueline de Souza [1] ; Pral, Lais Passariello [1] ; da Silva, Bruna Karadi [1] ; Correa, Renan Oliveira [1] ; Pereira de Andrade, Mirella Cristiny [1] ; da Fonseca, Denise Morais [2] ; Basso, Paulo Jose [2] ; Saraiva Camara, Niels Olsen [2] ; de Sales e Souza, Ericka Lorenna [3] ; Martins, Flaviano dos Santos [3] ; Sato Guima, Suzana Eiko [4] ; Thomas, Andrew Maltez [4] ; Setubal, Joao Carlos [4, 5] ; Magalhaes, Yuli Thamires [4] ; Forti, Fabio Luis [4] ; Candreva, Thamiris [6] ; Rodrigues, Hosana Gomes [6] ; de Jesus, Marcelo Bispo [7] ; Consonni, Silvio Roberto [8] ; Farias, Alessandro dos Santos [9] ; Varga-Weisz, Patrick [10, 11] ; Ramirez Vinolo, Marco Aurelio [1]
Total Authors: 23
Affiliation:
Show less -
[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Lab Immunoinflammat, BR-13083862 Campinas, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508900 Sao Paulo, SP - Brazil
[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Lab Biotherapeut Agents, BR-31270901 Belo Horizonte, MG - Brazil
[4] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil
[5] Virginia Polytech Inst & State Univ, Biocomplex Inst, Blacksburg, VA 24061 - USA
[6] Univ Estadual Campinas, Sch Appl Sci, Lab Nutrients & Tissue Repair, BR-13484350 Limeira, SP - Brazil
[7] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Nanocell Interact Lab, BR-13083862 Campinas, SP - Brazil
[8] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Citochem & Immunocitochem, BR-13083862 Campinas, SP - Brazil
[9] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Lab Neuroimmunol, BR-13083862 Campinas, SP - Brazil
[10] Univ Essex, Sch Biol Sci, Colchester CO4 3SQ, Essex - England
[11] Babraham Inst, Nucl Dynam Programme, Cambridge CB22 3AT - England
Total Affiliations: 11
Document type: Journal article
Source: CELL REPORTS; v. 27, n. 3, p. 750+, APR 16 2019.
Web of Science Citations: 3
Abstract

Antibiotic-induced dysbiosis is a key factor predisposing intestinal infection by Clostridium difficile. Here, we show that interventions that restore butyrate intestinal levels mitigate clinical and pathological features of C. difficile-induced colitis. Butyrate has no effect on C. difficile colonization or toxin production. However, it attenuates intestinal inflammation and improves intestinal barrier function in infected mice, as shown by reduced intestinal epithelial permeability and bacterial translocation, effects associated with the increased expression of components of intestinal epithelial cell tight junctions. Activation of the transcription factor HIF-1 in intestinal epithelial cells exerts a protective effect in C. difficile-induced colitis, and it is required for butyrate effects. We conclude that butyrate protects intestinal epithelial cells from damage caused by C. difficile toxins via the stabilization of HIF-1, mitigating local inflammatory response and systemic consequences of the infection. (AU)

FAPESP's process: 15/01507-7 - Microbial community profiling of human cancers
Grantee:Andrew Maltez Thomas
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/10653-9 - Role of short chain fatty acids and their receptor (GPR43) in the immune response to anaerobic bacteria in vivo and in vitro
Grantee:Marco Aurélio Ramirez Vinolo
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/06810-4 - Mechanisms of action of omega-3 and omega-6 in the tissue repair process: neuro-immune focus
Grantee:Hosana Gomes Rodrigues
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 17/25679-7 - Effects of short-chain fatty acids and their receptor FFAR2 on the development of periodontitis in an experimental model
Grantee:Bruna Karadi da Silva
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/01753-6 - Identification and functional investigation of proteins that interact with Cdc42 and DUSP12 enzymes in human cells under conditions of genomic instability: a proteomic approach
Grantee:Deborah Schechtman
Support type: Regular Research Grants
FAPESP's process: 17/16280-3 - Role of the interaction between HIF-1 and the short chain fatty acids in the colon in physiological and pathologycal conditions (intestinal inflammation)
Grantee:Marco Aurélio Ramirez Vinolo
Support type: Regular Research Grants
FAPESP's process: 17/06577-9 - Mechanisms involved in the protective effect of short-chain fatty acids against Clostridium difficile-associated colitis
Grantee:José Luís Fachi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/26366-2 - Influence of the inflammatory response on the extracellular matrix composition during the wound healing in fat-1 mice
Grantee:Thamiris Candreva Robles
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/23142-3 - "interaction between HIF-1 and short-chain fatty acids in the intestine: what is the role of HIF-1 acetylation? "
Grantee:Renan Oliveira Corrêa
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/03002-7 - Internalization and intracellular trafficking of nanoparticles: biological activity and nanotoxicological profile
Grantee:Marcelo Bispo de Jesus
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 15/06134-4 - Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader
Grantee:Marcelo Bispo de Jesus
Support type: Multi-user Equipment Program
FAPESP's process: 17/01451-7 - Investigating Rho GTPases pathways in the response of glioma cell lines to genotoxic stress
Grantee:Yuli Thamires Magalhães
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/02208-1 - Effect of short chain fatty acids on the function of type 3 innate lymphoid cells: involvement of the FFAR2 receptor in this process
Grantee:Laís Passariello Pral
Support type: Scholarships in Brazil - Master