Microbial community profiling of human cancers

• Grant number: 15/01507-7
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2015
• Effective date (End): December 31, 2018
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: João Carlos Setubal
• Grantee: Andrew Maltez Thomas
• Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 16/23527-2 - Identification of microbial biomarkers for colorectal cancer using strain-level metagenomic profiling, BE.EP.DR

Abstract

The microbial mass present in the human species is ten times greater than that of human cells. We know now that this metagenome influences us greatly, playing a role in our metabolism, tissue development, inflammation and immunity. Certain body habitats suffer greater exposure to the outside world than others, increasing the importance of the human microbiome on such body sites. Studying the microbial profiles associated with these habitats may lead to a greater understanding of diseases caused by infectious agents, including cancer, because estimates show that microbes play a central role in about 20% of all malignancies. To study the relationship between cancer and the human microbiome we plan to: 1) characterize the bacterial diversity present in gastric juice samples from subjects with pre-malignant lesions (n=30) or with gastric cancer (n=40), compared to subjects with no relevant clinical alterations (n=30); 2) characterize the bacterial diversity present in biopsy samples of subjects with rectal adenocarcinomas (n=18) and compare them to biopsy samples of subjects with no relevant clinical alterations (n=18); and 3) evaluate temporal alterations of bacterial communities caused by the use of radiation therapy using biopsy samples of colorectal cancer patients (n=40) across 3 times points to determine if they play a role in the development of proctitis. To do so, we will use a multi-disciplinary approach that will make use of genomic aspects, such as deep sequencing of the 16S rRNA bacterial gene, and advanced bioinformatics aspects to analyse the sequencing results. At the same time, we plan to mine public sequence databases using a bioinformatics pipeline in order to assess the presence of bacterial DNA or RNA, trying to validate findings obtained previously from the 16S rRNA gene. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AL ASSAL, KARINA; PRIFTI, EDI; BELDA, EUGENI; SALA, PRISCILA; CLEMENT, KARINE; DAO, MARIA-CARLOTA; DORE, JOEL; LEVENEZ, FLORENCE; TADDEI, CARLA R.; FONSECA, DANIELLE CRISTINA; ROCHA, ILANNA MARQUES; BALMANT, BIANCA DEPIERI; THOMAS, ANDREW MALTEZ; SANTO, MARCO A.; DIAS-NETO, EMMANUEL; SETUBAL, JOAO CARLOS; ZUCKER, JEAN-DANIEL; BELARMINO, GILIANE; TORRINHAS, RAQUEL SUSANA; WAITZBERG, DAN L. Gut Microbiota Profile of Obese Diabetic Women Submitted to Roux-en-Y Gastric Bypass and Its Association with Food Intake and Postoperative Diabetes Remission. NUTRIENTS, v. 12, n. 2 FEB 2020. Web of Science Citations: 2.

FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; MARTINS, FLAVIANO DOS SANTOS; SATO GUIMA, SUZANA EIKO; THOMAS, ANDREW MALTEZ; SETUBAL, JOAO CARLOS; MAGALHAES, YULI THAMIRES; FORTI, FABIO LUIS; CANDREVA, THAMIRIS; RODRIGUES, HOSANA GOMES; DE JESUS, MARCELO BISPO; CONSONNI, SILVIO ROBERTO; FARIAS, ALESSANDRO DOS SANTOS; VARGA-WEISZ, PATRICK; RAMIREZ VINOLO, MARCO AURELIO. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, APR 16 2019. Web of Science Citations: 3.

THOMAS, ANDREW M.; JESUS, ELIANE C.; LOPES, ADEMAR; AGUIAR, JR., SAMUEL; BEGNAMI, MARIA D.; ROCHA, RAFAEL M.; CARPINETTI, PAOLA AVELAR; CAMARGO, ANAMARIA A.; HOFFMANN, CHRISTIAN; FREITAS, HELANO C.; SILVA, ISRAEL T.; NUNES, DIANA N.; SETUBAL, JOAO C.; DIAS-NETO, EMMANUEL. Tissue-Associated Bacterial Alterations in Rectal Carcinoma Patients Revealed by 16S rRNA Community Profiling. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 6, DEC 9 2016. Web of Science Citations: 19.

KINKER, GABRIELA SARTI; THOMAS, ANDREW MALTEZ; CARVALHO, VINICIUS JARDIM; LIMA, FELIPE PRATA; FUJITA, ANDRE. Deletion and low expression of NFKBIA are associated with poor prognosis in lower-grade glioma patients. SCIENTIFIC REPORTS, v. 6, APR 7 2016. Web of Science Citations: 4.