Effects of short-chain fatty acids and their receptor FFAR2 on the development of periodontitis in an experimental model

• Grant number: 17/25679-7
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2018
• Effective date (End): February 29, 2020
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Marco Aurélio Ramirez Vinolo
• Grantee: Bruna Karadi da Silva
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Periodontitis is a chronic inflammatory disease that affects millions of people around the world. Its main characteristics are a dysbiosis in the oral microbiota and recruitment of immune cells to the periodontal tissue, that results in the destruction of tooth support tissues and bone loss. It is evident in recent studies that the intestinal microbiota regulates inflammation in different tissues and may affect bone homeostasis. However, little is known about the relationship between the intestinal microbiota and periodontal disease, in particular to the molecular mechanisms involved. In this sense, this research aims to evaluate the action of intestinal microbiota metabolism products, the short chain fatty acids (SCFA), and its associated receptor (FFAR2) in murine experimental periodontitis development. To this end, the ligature method will be used to induce periodontitis in mice (which consists of implementing a suture line around the mice's first molar) coupled with strategies that upregulates and downregulates the systemic concentration of SCFA, including the ministration of this compounds in the drinking water, diets with and without fibers (SCFA intestinal generation source) and intestinal microbiota depletion with antibiotics. Furthermore, experiments will be conducted with both mice that expresses the SCFA receptor (FFAR2+/+) and do not express (FFAR2-/-) and also be considered the ones that expresses it in all of its cells or only in its hematopoietic cells (bone marrow chimaera experiments). Results will be analyzed in terms of alveolar bone loss, gingival and submandibular lymph nodes inflammatory markers and leukocyte population. SCFA concentration in the mice's blood stream and at luminal content will also be measured and correlated to the disease intensity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FACHI, JOSE LUIS; FELIPE, JAQUELINE DE SOUZA; PRAL, LAIS PASSARIELLO; DA SILVA, BRUNA KARADI; CORREA, RENAN OLIVEIRA; PEREIRA DE ANDRADE, MIRELLA CRISTINY; DA FONSECA, DENISE MORAIS; BASSO, PAULO JOSE; SARAIVA CAMARA, NIELS OLSEN; DE SALES E SOUZA, ERICKA LORENNA; MARTINS, FLAVIANO DOS SANTOS; SATO GUIMA, SUZANA EIKO; THOMAS, ANDREW MALTEZ; SETUBAL, JOAO CARLOS; MAGALHAES, YULI THAMIRES; FORTI, FABIO LUIS; CANDREVA, THAMIRIS; RODRIGUES, HOSANA GOMES; DE JESUS, MARCELO BISPO; CONSONNI, SILVIO ROBERTO; FARIAS, ALESSANDRO DOS SANTOS; VARGA-WEISZ, PATRICK; RAMIREZ VINOLO, MARCO AURELIO. Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism. CELL REPORTS, v. 27, n. 3, p. 750+, APR 16 2019. Web of Science Citations: 3.