he effect of short chain fatty acids and its receptor FFAR2 on periodontitis development in a experimental model

Author(s)

Bruna Karadi da Silva

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2020-08-10

Examining board members

Marco Aurélio Ramirez Vinolo; Sandra Yasuyo Fukada Alves; José Luiz Proença Módena

• Advisor: Marco Aurélio Ramirez Vinolo

Abstract

Periodontitis is a chronic inflammatory disease that afflicts millions around the world. It is characterized by a disbiosis in the oral microbiota which increases the recruitment and activation of immune cells at periodontal tissues, leading to periodontal bone loss. Recent literature suggests that gut microbiota have an important role regulating exacerbated inflammation and also display positive effect on bone loss, such as the femur. However, the influence of gut microbiota on periodontitis development, especially considering the molecular mechanisms involved, still a gap in the literature. The aim of this study is to evaluate the effects of the gut microbiota¿s molecular products, the Short Chain Fatty Acids (SCFA) and its receptor FFAR2, on periodontitis in mice. As methodological procedure we used the ligature-induced periodontitis experimental model coupled with strategies to upregulate and downregulate the gut and systemic SCFA concentration. Furthermore, we used mice that expresses the SCFA receptor (FFAR2+/+) and Knockout mice for the SCFA receptor (FFAR2-/-). The results show that when the SCFA endogenous production decreases, through low-fiber diets or through depleting gut microbiota with antibiotics, periodontal bone loss increases. Hence, showing a deleterious effect. Our results also indicate that bone loss is greater in the absence of the SCFA receptor, an effect connected to a decrease in neutrophilic infiltrate at the gum. Our findings highlight the importance of gut microbiota and the SCFA on bone homeostasis (AU)

• FAPESP's process: 17/25679-7 - Effects of short-chain fatty acids and their receptor FFAR2 on the development of periodontitis in an experimental model
• Grantee: Bruna Karadi da Silva
• Support Opportunities: Scholarships in Brazil - Master