Paper platform for determination of bumetanide in human urine samples to detect doping in sports using digital image analysis

Autor(es)

Marques Luiz, Vitor Hugo ^[1] ; Lima, Liliane Spazzapam ^[1] ; Rossini, Eduardo Luiz ^[1] ; Pezza, Leonardo ^[1] ; Pezza, Helena Redigolo ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Univ Estadual Paulista Julio de Mesquita Filho, Inst Quim, R Prof Francisco Degni 55, POB 355, BR-14800900 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 147, p. 43-48, JUN 2019.
• Citações Web of Science: 2

Resumo

Development and application of a new, simple, inexpensive, and eco-friendly method is presented for the quantification of bumetanide in human urine samples for the detection of doping in sports. The method involves the use of a paper platform and digital image detection and is based on the reaction between bumetanide, p-dimethylaminocinnamaldehyde (p-DAC), and HCl in a methanolic medium, yielding a colored compound in a delimited area on a qualitative filter paper. The concentrations of p-DAC and HCl were optimized by a chemometric experimental design. After the addition of the reagents to the paper, digital images were obtained by scanning in a multifunctional printer and analyzed using the RGB pattern. The linear bumetanide concentration range was 6.90 x 10(-5) -1.37 x 10(-3) mol L-1 with R-2 = 0.993 and the limits of detection (LOD) and quantification (LOQ) were 3.60 x 10(-4) and 1.09 x 10(-4) mol L-1, respectively. A SPE clean-up step for the samples were required to eliminate the interference of urinary urea. The recoveries obtained varied from 94% to 105%, indicating the absence of significant matrix effects or interferences in urine samples after clean-up step. The proposed method was successfully applied to the analysis of bumetanide in human urine samples. (AU)

• Processo FAPESP: 15/21733-1 - Desenvolvimento de métodos analíticos de vanguarda utilizando spot test e dispositivo microfluidico em papel.
• Beneficiário: Helena Redigolo Pezza
• Modalidade de apoio: Auxílio à Pesquisa - Regular