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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Paper platform for determination of bumetanide in human urine samples to detect doping in sports using digital image analysis

Full text
Author(s):
Marques Luiz, Vitor Hugo [1] ; Lima, Liliane Spazzapam [1] ; Rossini, Eduardo Luiz [1] ; Pezza, Leonardo [1] ; Pezza, Helena Redigolo [1]
Total Authors: 5
Affiliation:
[1] UNESP, Univ Estadual Paulista Julio de Mesquita Filho, Inst Quim, R Prof Francisco Degni 55, POB 355, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Microchemical Journal; v. 147, p. 43-48, JUN 2019.
Web of Science Citations: 2
Abstract

Development and application of a new, simple, inexpensive, and eco-friendly method is presented for the quantification of bumetanide in human urine samples for the detection of doping in sports. The method involves the use of a paper platform and digital image detection and is based on the reaction between bumetanide, p-dimethylaminocinnamaldehyde (p-DAC), and HCl in a methanolic medium, yielding a colored compound in a delimited area on a qualitative filter paper. The concentrations of p-DAC and HCl were optimized by a chemometric experimental design. After the addition of the reagents to the paper, digital images were obtained by scanning in a multifunctional printer and analyzed using the RGB pattern. The linear bumetanide concentration range was 6.90 x 10(-5) -1.37 x 10(-3) mol L-1 with R-2 = 0.993 and the limits of detection (LOD) and quantification (LOQ) were 3.60 x 10(-4) and 1.09 x 10(-4) mol L-1, respectively. A SPE clean-up step for the samples were required to eliminate the interference of urinary urea. The recoveries obtained varied from 94% to 105%, indicating the absence of significant matrix effects or interferences in urine samples after clean-up step. The proposed method was successfully applied to the analysis of bumetanide in human urine samples. (AU)

FAPESP's process: 15/21733-1 - Development of vanguard analytical methods using spot test and paper-based microfluidic device
Grantee:Helena Redigolo Pezza
Support type: Regular Research Grants