Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Autor(es)

Sper Simao, Ana Maria ^[1] ; Bolean, Mayte ^[1] ; Favarin, Bruno Zoccaratto ^[1] ; Veschi, Ekeveliny Amabile ^[1] ; Tovani, Camila Bussola ^[1] ; Ramos, Ana Paula ^[1] ; Bottini, Massimo ^{[2, 3]} ; Buchet, Rene ^{[4, 5, 6, 7, 8]} ; Millan, Jose Luis ^[2] ; Ciancaglini, Pietro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FFCLRP USP, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA

^[3] Univ Roma Tor Vergata, Dept Expt Med & Surg, I-00133 Rome - Italy

^[4] Univ Lyon, F-69622 Villeurbanne - France

^[5] Univ Lyon 1, UFR Chim Biochim, F-69622 Villeurbanne - France

^[6] INSA, F-69622 Villeurbanne - France

^[7] CPE, F-69622 Villeurbanne - France

^[8] ICBMS, CNRS, UMR 5246, F-69622 Villeurbanne - France

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BONE AND MINERAL METABOLISM; v. 37, n. 4, p. 607-613, JUL 2019.
• Citações Web of Science: 3

Resumo

Tissue-nonspecific alkaline phosphatase (TNAP), a glycosylphosphatidylinositol-anchored ectoenzyme present on the membrane of matrix vesicles (MVs), hydrolyzes the mineralization inhibitor inorganic pyrophosphate as well as ATP to generate the inorganic phosphate needed for apatite formation. Herein, we used proteoliposomes harboring TNAP as MV biomimetics with or without nucleators of mineral formation (amorphous calcium phosphate and complexes with phosphatidylserine) to assess the role of the MVs' membrane lipid composition on TNAP activity by means of turbidity assay and FTIR analysis. We found that TNAP-proteoliposomes have the ability to induce mineralization even in the absence of mineral nucleators. We also found that the addition of cholesterol or sphingomyelin to TNAP-proteoliposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine reduced the ability of TNAP to induce biomineralization. Our results suggest that the lipid microenvironment is essential for the induction and propagation of minerals mediated by TNAP. (AU)

• Processo FAPESP: 14/11941-3 - Colágeno e annexina são responsáveis pelo controle do processo de biomineralização?
• Beneficiário: Pietro Ciancaglini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/21236-0 - Sistemas miméticos de vesículas da matriz extracellular (MVs) para o estudo da regulação do processo de biomineralização: proteolipossomos contendo NPP1 e Anexina V
• Beneficiário: Pietro Ciancaglini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/00371-1 - Interações entre colágeno e proteínas/enzimas presentes nas vesículas da matriz são responsáveis pelo controle do processo de biomineralização?
• Beneficiário: Maytê Bolean Correia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/08892-9 - Superfícies bioativas obtidas a partir de filmes Langmuir-Blodgett e biominerais
• Beneficiário: Ana Paula Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular