Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Author(s)

Sper Simao, Ana Maria ^[1] ; Bolean, Mayte ^[1] ; Favarin, Bruno Zoccaratto ^[1] ; Veschi, Ekeveliny Amabile ^[1] ; Tovani, Camila Bussola ^[1] ; Ramos, Ana Paula ^[1] ; Bottini, Massimo ^{[2, 3]} ; Buchet, Rene ^{[4, 5, 6, 7, 8]} ; Millan, Jose Luis ^[2] ; Ciancaglini, Pietro ^[1]

Affiliation

^[1] Univ Sao Paulo FFCLRP USP, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA

^[3] Univ Roma Tor Vergata, Dept Expt Med & Surg, I-00133 Rome - Italy

^[4] Univ Lyon, F-69622 Villeurbanne - France

^[5] Univ Lyon 1, UFR Chim Biochim, F-69622 Villeurbanne - France

^[6] INSA, F-69622 Villeurbanne - France

^[7] CPE, F-69622 Villeurbanne - France

^[8] ICBMS, CNRS, UMR 5246, F-69622 Villeurbanne - France

• Document type: Journal article
• Source: JOURNAL OF BONE AND MINERAL METABOLISM; v. 37, n. 4, p. 607-613, JUL 2019.
• Web of Science Citations: 3

Abstract

Tissue-nonspecific alkaline phosphatase (TNAP), a glycosylphosphatidylinositol-anchored ectoenzyme present on the membrane of matrix vesicles (MVs), hydrolyzes the mineralization inhibitor inorganic pyrophosphate as well as ATP to generate the inorganic phosphate needed for apatite formation. Herein, we used proteoliposomes harboring TNAP as MV biomimetics with or without nucleators of mineral formation (amorphous calcium phosphate and complexes with phosphatidylserine) to assess the role of the MVs' membrane lipid composition on TNAP activity by means of turbidity assay and FTIR analysis. We found that TNAP-proteoliposomes have the ability to induce mineralization even in the absence of mineral nucleators. We also found that the addition of cholesterol or sphingomyelin to TNAP-proteoliposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine reduced the ability of TNAP to induce biomineralization. Our results suggest that the lipid microenvironment is essential for the induction and propagation of minerals mediated by TNAP. (AU)

• FAPESP's process: 14/11941-3 - Are collagen and Annexin V responsible for the control in the biomineralization process?
• Grantee: Pietro Ciancaglini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/21236-0 - Extracellular matrix vesicles (MVs) mimetic systems to study the regulation of the biomineralization process: proteoliposomes containing NPP1 and Annexin V
• Grantee: Pietro Ciancaglini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/00371-1 - Are the interactions between collagen and proteins/enzymes present in the matriz vesicles responsible for the control in the biomineralization process?
• Grantee: Maytê Bolean Correia
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/08892-9 - Bioactive surfaces designed from Langmuir-Blodgett Films and Biominerals
• Grantee: Ana Paula Ramos
• Support Opportunities: Regular Research Grants