Development and characterization of membranes derived from SF/GLY/58S hybrid xerogels for the release of inorganic ions as an osteogenic stimulus for bone regeneration

Autor(es)

Lopes, J. H. ^{[1, 2]} ; Franca, C. G. ^[1] ; Beppu, M. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Sch Chem Engn FEQ, Lab Engn & Prod Chem LEQUIP, Dept Mat Engn & Biopr, BR-13083852 Campinas, SP - Brazil

^[2] Aeronaut Inst Technol ITA, Div Fundamental Sci IEF, Dept Chem, Praca Marechal Eduardo Gomes 50, BR-12228900 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN POLYMER JOURNAL; v. 116, p. 425-437, JUL 2019.
• Citações Web of Science: 0

Resumo

The ternary membrane-forming system based on the silk fibroin (SF), glycerol (GLY) and sol-gel precursor of 58S bioactive glass (BG) was studied for the preparation of novel and multifunctional hybrid membranes. The morphology of membranes was observed by scanning electron microscopy (SEM) and spectral imaging, by X-ray mapping technique, were performed in order to investigate the chemical homogeneity of elements. The results show that there is a critical limit for the concentration of GLY and BG for formation of flexible membranes and without a phase separation. XRD and FTIR data showed that the GLY and BG in the silk matrix modifies the short- and intermediate-range order resulting in changes in the silk conformation, i.e., SF molecules are gradually transformed from random coils toward more stable structures - Silk I and Silk II (beta-sheets). The solubility study showed that the hybrid membranes have an excellent chemical stability, confirming that the incorporation of inorganic species in the matrix allowed the preparation of novel well-controlled water-soluble membranes. The release profiles of Ca, P and Si confirmed ability of hybrid membranes to deliver controlled inorganic species, which in critical concentration, control the gene expression of osteoblasts and influences cell metabolism and function. (AU)

• Processo FAPESP: 10/05394-9 - Biovidros derivados do 45S5: os efeitos do Nb2O5 ou da modificação da superfície com Ca2+ sobre a estrutura e bioatividade
• Beneficiário: João Henrique Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado