| Texto completo | |
| Autor(es): |
Mestre, Julia C.
[1]
;
Cerri, Marcel O.
[2]
;
Esperanca, Mateus N.
[3]
;
Pedrolli, Danielle B.
[2]
;
Badino, Alberto C.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[2] Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara - Brazil
[3] Fed Inst Educ Sci & Technol Sao Paulo, Capivari - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY; v. 94, n. 7, p. 2327-2332, JUL 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Background Determination of the volumetric oxygen transfer coefficient (k(L)a) plays an important role in the design and characterization of bioreactors. Among all methods available, few techniques are suitable for the determination of k(L)a in pneumatic bioreactors during the course of cultivations. The present work proposes a new aeration step method for k(L)a evaluation in pneumatic bioreactors during biological consumption of oxygen. Results The aeration step method consisted in promoting step changes in the specific air flow rate (phi(air)) to change the dissolved oxygen concentration during cultivations, allowing the measurement of both overall oxygen transfer coefficient (k(L)a) and specific oxygen uptake rate (Q(O2)). These parameters were determined experimentally by employing two microorganisms, one a strict aerobe (Bacillus subtilis) and the other a facultative aerobe (Saccharomyces cerevisiae), for phi(air) values ranging from 2.0 to 5.0 vvm. For the purpose of comparison, k(L)a was also determined in experiments using water as the liquid phase, employing a dynamic gassing-out method. The k(L)a values during the cultivations ranged from 0.0137 to 0.0545 s(-1), while Q(O2) varied from 0.44 to 10.06 mol kg(-1) h(-1). The results obtained are shown to be reliable, exhibited excellent reproducibility and are in agreement with other values reported in the literature by different methodologies. Conclusion The new aeration step method proved to be reliable as an alternative technique for the determination of k(L)a and Q(O2) during microbial cultivation in pneumatic bioreactors. (c) 2019 Society of Chemical Industry (AU) | |
| Processo FAPESP: | 11/23807-1 - Desenvolvimento e avaliação das condições de operação de biorreatores visando a produção de produtos biotecnológicos |
| Beneficiário: | Alberto Colli Badino Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/11405-5 - Fermentação Alcoólica Extrativa em Biorreatores Pneumáticos com Arraste de Etanol por CO2 Integrada a Etapas de Recuperação |
| Beneficiário: | Alberto Colli Badino Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/17756-8 - Avaliação das condições hidrodinâmicas, de cisalhamento e de transferência de oxigênio de diferentes modelos de biorreatores pneumáticos |
| Beneficiário: | Caroline Eliza Mendes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |