Bioactivity and action mechanism of green propolis against Pythium aphanidermatum

Autor(es)

Pazin, Wallance M. ^{[1, 2]} ; Dos Santos, Suikinai N. ^[3] ; Queiroz, Sonia C. N. ^[3] ; Bagatolli, Luis A. ^[4] ; Soares, Ademilson E. E. ^[5] ; De Melo, Itamar S. ^[3] ; Ito, Amando S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Fis, Rua Roberto Simonsen 305, BR-19060900 Presidente Prudente, SP - Brazil

^[3] Embrapa Meio Ambiente, Lab Microbiol Ambiental, Rodovia SP 340, Km 127, 5, BR-13918110 Jaguariuna, SP - Brazil

^[4] UNC, CONICET, INIMEC, Inst Invest Med Mercedes & Martin Ferreyra, RA-2434 Cordoba - Argentina

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anais da Academia Brasileira de Ciências; v. 91, n. 2 2019.
• Citações Web of Science: 0

Resumo

Abstract: We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture. (AU)

• Processo FAPESP: 16/09633-4 - Mecanismo de ação do composto bioativo Artepillin C em sistemas miméticos de membranas e membranas citoplasmáticas de células tumorais
• Beneficiário: Wallance Moreira Pazin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado