Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae)

Autor(es)

de Russo Godoy, Fernanda Maria ^[1] ; Paggi, Gecele Matos ^{[1, 2]} ; Palma-Silva, Clarisse ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Mato Grosso do Sul, Fac Ciencias Farmaceut Alimentos & Nutr, Programa Posgrad Biotecnol & Biodiversidad, BR-79070900 Campo Grande, MS - Brazil

^[2] Univ Fed Mato Grosso do Sul, Ciencias Biol, Campus Pantanal, BR-79304902 Corumba, MS - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Rev. bras. Bot.; v. 42, n. 2, p. 353-359, JUN 2019.
• Citações Web of Science: 0

Resumo

Aechmea distichantha Lem. is a Bromeliaceae species with wide geographic distribution; it is found in Atlantic Forest, ``Cerrado{''} and ``Chaco{''} ecoregions from Tropical to Subtropical areas in Brazil, Bolivia, Paraguay and Argentina. The species has a great ornamental value and is currently threatened by its predatory exploration and by habitat loss. The lack of suitable molecular markers hampers research on its genetic diversity, which could contribute to the design of conservation plans and to demographic and phylogeographic studies of the species. Here, we tested the cross-amplification of 43 nuclear microsatellite markers, originally developed for other bromeliad species. We obtained high cross-amplification indices (37 SSR primers, 86%) and polymorphism (17 SSR primers, 46%). We then used ten SSR loci to genotype individuals from three populations of A. distichantha. The observed and expected heterozygosity per locus in the A. distichantha populations ranged from 0.182 to 0.735 and 0.297 to 0.830, respectively. These loci showed sufficient variability to be used in future the studies of genetic diversity, genetic structure and phylogeography of A. distichantha to understand its evolutionary history during its dispersal, colonization and adaptation to different ecoregions. (AU)

• Processo FAPESP: 18/07596-0 - Filogeografia, genômica populacional e especiação de populações naturalmente fragmentadas de Bromeliaceae
• Beneficiário: Clarisse Palma da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular