Genetic diversity of Hepatozoon spp. in rodents from Brazil

Autor(es)

Perles, L. ^[1] ; Roque, A. L. R. ^[2] ; D'Andrea, P. S. ^[3] ; Lemos, E. R. S. ^[4] ; Santos, A. F. ^[5] ; Morales, A. C. ^[5] ; Machado, R. Z. ^[1] ; Andre, M. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, FCAV, UNESP, Lab Imunoparasitol, Dept Patol Vet, Jaboticabal, SP - Brazil

^[2] Inst Oswaldo Cruz, Lab Biol Tripanosomatideos, FIOCRUZ, Rio De Janeiro, RJ - Brazil

^[3] Inst Oswaldo Cruz, Lab Biol & Parasitol Mamiferos Silvestres Reserva, FIOCRUZ, Rio De Janeiro, RJ - Brazil

^[4] Inst Oswaldo Cruz, Lab Hantaviroses & Rickettsioses, FIOCRUZ, Rio De Janeiro, RJ - Brazil

^[5] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, UNESP, Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JUL 12 2019.
• Citações Web of Science: 1

Resumo

Hepatozoon spp. are Apicomplexan protozoa that parasitize a wide diversity of vertebrate hosts. In Brazil, few studies have reported the occurrence of Hepatozoon spp. in rodent species. Additionally, an evaluation of the population structure and distribution of Hepatozoon species over several Brazilian biomes has not yet been performed. The present work aimed to investigate the genetic diversity of Hepatozoon spp. in rodents from 31 genera sampled in five Brazilian biomes. Samples were submitted to PCR assays for Hepatozoon spp. targeting two regions of the 18S rRNA gene. Infection by Hepatozoon spp. was detected in 195 (42.2%) rodents comprising 24 genera. Phylogenetic analyses of 18S rRNA sequences grouped all sequences in the clade of Hepatozoon spp. previously detected in rodents and reptiles, apart from those detected in domestic/wild carnivores. These data raise two non-exclusive hypotheses: (i) rodents play an important role as intermediate or paratenic hosts for Hepatozoon infections in reptiles; and (ii) rodents do not seem to participate in the epidemiology of Hepatozoon infections of domestic/wild canids and felids in Brazil. TCS analyses performed with available 18S rRNA Hepatozoon sequences detected in rodents from Brazil showed the occurrence of six haplotypes, which were distributed in two large groups: one from rodents inhabiting the coastal region of Brazil and Mato Grosso state, and another from rodents from the central region of the country. A wide survey of the South American territory will help to elucidate the evolutionary history of Hepatozoon spp. parasitizing Rodentia in the American continent. (AU)

• Processo FAPESP: 15/14896-1 - Detecção e caracterização molecular de agentes das famílias Anaplasmataceae e Bartonellaceae em animais selvagens e ectoparasitas no Brasil
• Beneficiário: Marcos Rogério André
• Modalidade de apoio: Auxílio à Pesquisa - Regular