Gonadal, body color, and genotoxic alterations in Lithobates catesbeianus tadpoles exposed to nonylphenol

Autor(es)

Scaia, Maria Florencia ^{[1, 2, 3, 4]} ; de Gregorio, Lara Salgueiro ^[1] ; Franco-Belussi, Lilian ^{[4, 5]} ; Succi-Domingues, Maysa ^[4] ; de Oliveira, Classius ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Postgrad Program Anim Biol, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Consejo Nacl Invest Cient & Tecn, Inst Biodiversidad Biol Expt & Aplicada, C1428EGA, Buenos Aires, DF - Argentina

^[3] Univ Buenos Aires, Lab Neuroendocrinol & Comportamiento, Dept Biodiversidad & Biol Expt, Fac Ciencias Exactas & Nat, C1428EGA, Buenos Aires, DF - Argentina

^[4] Inst Biociencias Letras & Ciencias Exatas UNESP I, Dept Biol, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Fed Mato Grosso Sul UFMS, Inst Biociencias InBio, Campo Grande - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 26, n. 22, p. 22209-22219, AUG 2019.
• Citações Web of Science: 0

Resumo

Endocrine disrupting chemicals are one of the most important factors contributing to worldwide amphibian decline. The 4-nonylphenol (NP) is a degradation product of several compounds, such as detergents and pesticides, affecting the aquatic environment. Here, we test whether treatment with NP has an effect on developing ovarian tissue, nuclear abnormalities in erythrocytes, and body darkness in pre-metamorphic tadpoles of the bullfrog Lithobates catesbeianus. Tadpoles were exposed for 14days to three different concentrations of NP (1, 10, and 100 mu g/L) besides the control group, which was maintained only with water. After determining body coloration, animals were euthanized and gonads and blood were collected and processed for histology and genotoxic analysis. Even though most animals were females, intersex tadpoles were observed in control and treated groups and there were no males in any group. The highest concentration of NP showed an increase in atretic oocytes, but the area corresponding to somatic compartment and early and late germ cells were not affected. Furthermore, all treated groups presented higher amount of nuclear abnormalities in erythrocytes and body darkening when compared with the control group. These results suggest that NP causes genetic damage and morphological alterations in L. catesbeianus tadpoles by disrupting oogenesis, inducing genotoxicity and increasing body coloration. Its effects on gonadal development could cause future impairments in reproduction, while its deleterious effects on genotoxicity and body pigmentation could be used as a biomarker of effect to this compound. (AU)

• Processo FAPESP: 14/00946-4 - Pigmentação Visceral em Animais Ectotérmicos: Imunidade Inata e Defesa contra UV
• Beneficiário: Lilian Franco Belussi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/12006-9 - Influência de contaminantes ambientais (Acetato de ciproterona e Nonilfenol) e fatores abióticos (Temperatura e Radiação UV) na Melanina interna em anfíbios anuros
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular