Incorporation of collagen and PLGA in bioactive glass: in vivo biological evaluation

Autor(es)

Paiva Magri, Angela Maria ^{[1, 2]} ; Fernandes, Kelly Rossetti ^[1] ; Assis, Livia ^[1] ; Kido, Hueliton Wilian ^[1] ; Avanzi, Ingrid Regina ^[1] ; Medeiros, Matheus da Cruz ^[1] ; Granito, Renata Neves ^[1] ; Correa Braga, Francisco Jose ^[3] ; Muniz Renno, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil

^[2] Univ Ctr Guaxupe Educ Fdn UNIFEG, Ave Dona Floriana, BR-37800000 Guaxupe, MG - Brazil

^[3] Consulmat LTDA, Rua Juan Lopes 159, BR-13567020 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 134, p. 869-881, AUG 1 2019.
• Citações Web of Science: 2

Resumo

Bioactive glasses (BG) are known for their unique ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not sufficient to produce bone consolidation. The use of composite materials may constitute an optimized therapeutical intervention for bone stimulation. The aim of this study was to characterize BG/collagen/poly (D,L-lactic-co-glycolic) acid (BG/COL/PLGA) composites, in vitro biocompatibility and in vivo biological properties. MC3T3-E1 cells were evaluated by cell proliferation, ALP activity, cell adhesion and morphology. Qualitative histology and immunohistochemistry were performed in a calvarial bone defect model in rats. The in vitro study demonstrated, after 3 and 6 days of culture, a significant increase of proliferation was observed for BG/PLGA compared to BG/COL and BG/COL/PLGA. BG/COL/PLGA presented a higher value for ALP activity after 3 days of culture compared to BG/PLGA. For in vivo analysis, 6 weeks post-surgery, BG/PLGA showed a more mature neoformed bone tissue. As a conclusion, the in vitro and in vivo studies pointed out that BG/PLGA samples improved biological properties in calvarial bone defects, highlighting the potential of BG/PLGA composites to be used as a bone graft for bone regeneration applications. (C) 2019 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/20546-0 - Laser terapêutico e compósitos de Biosilicato®/magnésio/colágeno, utilizados em associação ou isolados: avaliação biológica in vitro e in vivo no tecido ósseo
• Beneficiário: Ana Claudia Muniz Renno
• Modalidade de apoio: Auxílio à Pesquisa - Regular