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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Incorporation of collagen and PLGA in bioactive glass: in vivo biological evaluation

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Paiva Magri, Angela Maria [1, 2] ; Fernandes, Kelly Rossetti [1] ; Assis, Livia [1] ; Kido, Hueliton Wilian [1] ; Avanzi, Ingrid Regina [1] ; Medeiros, Matheus da Cruz [1] ; Granito, Renata Neves [1] ; Correa Braga, Francisco Jose [3] ; Muniz Renno, Ana Claudia [1]
Total Authors: 9
[1] Fed Univ Sao Paulo UNIFESP, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil
[2] Univ Ctr Guaxupe Educ Fdn UNIFEG, Ave Dona Floriana, BR-37800000 Guaxupe, MG - Brazil
[3] Consulmat LTDA, Rua Juan Lopes 159, BR-13567020 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 134, p. 869-881, AUG 1 2019.
Web of Science Citations: 2

Bioactive glasses (BG) are known for their unique ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not sufficient to produce bone consolidation. The use of composite materials may constitute an optimized therapeutical intervention for bone stimulation. The aim of this study was to characterize BG/collagen/poly (D,L-lactic-co-glycolic) acid (BG/COL/PLGA) composites, in vitro biocompatibility and in vivo biological properties. MC3T3-E1 cells were evaluated by cell proliferation, ALP activity, cell adhesion and morphology. Qualitative histology and immunohistochemistry were performed in a calvarial bone defect model in rats. The in vitro study demonstrated, after 3 and 6 days of culture, a significant increase of proliferation was observed for BG/PLGA compared to BG/COL and BG/COL/PLGA. BG/COL/PLGA presented a higher value for ALP activity after 3 days of culture compared to BG/PLGA. For in vivo analysis, 6 weeks post-surgery, BG/PLGA showed a more mature neoformed bone tissue. As a conclusion, the in vitro and in vivo studies pointed out that BG/PLGA samples improved biological properties in calvarial bone defects, highlighting the potential of BG/PLGA composites to be used as a bone graft for bone regeneration applications. (C) 2019 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/20546-0 - Lasertherapy and Biosilicate/magnesium/collagen composites, used in association or isolated: in vitro and in vivo biological evaluation in bone tissue
Grantee:Ana Claudia Muniz Renno
Support type: Regular Research Grants