Sequential high-pressure extraction to obtain capsinoids and phenolic compounds from biquinho pepper (Capsicum chinense)

Autor(es)

de Aguiar, Ana Carolina ^[1] ; da Fonseca Machado, Ana Paula ^[1] ; Figueiredo Angolini, Cello Fernando ^[2] ; de Morais, Damila Rodrigues ^[2] ; Baseggio, Andressa Mara ^[3] ; Eberlin, Marcos Nogueira ^[2] ; Marostica Junior, Mario R. ^[3] ; Martinez, Julian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Coll Food Engn, Dept Food Engn, R Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, R Josue de Castro, BR-13083970 Campinas, SP - Brazil

^[3] Univ Campinas UNICAMP, Coll Food Engn, Dept Food & Nutr, R Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SUPERCRITICAL FLUIDS; v. 150, p. 112-121, AUG 2019.
• Citações Web of Science: 3

Resumo

This work proposes a sequential high-pressure extraction to recover bioactives from biquinho peppers. The nonpolar fraction was extracted with supercritical carbon dioxide (sc-CO2). Then, the sc-CO2 extracted biquinho pepper (EBP) was submitted to pressurized liquid extraction (PLE) using ethanol and water mixtures (50%, 75%, 100% ethanol) at different temperatures (45, 55, 65 degrees C) to recover compounds of higher polarity. The sc-CO2 extraction yield was 4.75%, with capsiate concentration of 8.67 mg/g oleoresin. In PLE, the solvent composition influenced the extraction yield and quality. Sixteen phenolics were identified in PLE extracts by UHPLC-MS/MS. Rutin isomer and vicenin-2 were quantified. The highest concentration of rutin isomer (441 mu g/g extract) was obtained with pure ethanol whereas, for vicenin-2, the best solvent was 50% ethanol (299 mu g/g extract). Considering the extraction of total phenolics, 75% ethanol was the most effective solvent. sc-CO2 extraction followed by PLE is an interesting alternative to obtain bioactives from peppers. (C) 2019 Published by Elsevier B.V. (AU)

• Processo FAPESP: 15/18119-0 - Emulsificação por ultrassom de extratos de pimentas biquinho (Capsicum chinense) obtidos por extração supercrítica como alternativa para o aumento da bioatividade
• Beneficiário: Ana Carolina de Aguiar
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado