| Texto completo | |
| Autor(es): |
Zaninette, Fernanda
[1]
;
Lopes de Melo Rocha, Giulliana Aparecida
[1]
;
Bom Pessoni, Rosemeire Aparecida
[2]
;
Braga, Marcia Regina
[1]
;
Simoes, Kelly
[1]
;
Leone Figueiredo-Ribeiro, Rita de Cassia
[1]
;
Fialho, Mauricio Batista
[3]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, Sao Paulo - Brazil
[2] Univ Metodista Sao Paulo, Fac Saude, Sao Bernardo Do Campo - Brazil
[3] Univ Fed ABC, Prograd, Lab Biol, Ave Estados 5001, BR-09210580 Santo Andre, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Biotechnology and Applied Biochemistry; v. 66, n. 3, p. 419-425, MAY-JUN 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of beta-fructosyltransferases and/or beta-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in a medium supplemented with sucrose concentrations of 30, 100, or 150 g L-1. Hydrolytic activity on sucrose was observed in culture filtrates; however, at 150 g L-1 sucrose, the accumulation of 8 g L-1 1-kestose (inulin-type FOS) and 7.3 g L-1 neokestose (neolevan-type FOS) was observed, the latter being a type of FOS not commonly produced by filamentous fungi. In addition, minor amounts of four unidentified oligosaccharides, with a high degree of polymerization, were detected. The production of FOS was also observed in enzymatic assays, indicating the presence of extracellular enzymes with transfructosylating activity in the culture filtrates. Our findings demonstrate the feasibility of isolating promising microorganisms, for the production of FOS-synthesizing enzymes, from the rhizosphere of fructan-producing plants of the Brazilian Cerrado. (AU) | |
| Processo FAPESP: | 09/06171-6 - Influência de diferentes fontes de carbono na ultra-estrutura e composição da parede celular e na produção e expressão de invertases e inulinases em Penicillium janczewskii Zaleski |
| Beneficiário: | Kelly Simões |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 05/04139-7 - Carboidratos de plantas tropicais como moduladores de processos ecofisiológicos e indicadores de respostas a estresses ambientais |
| Beneficiário: | Marcia Regina Braga |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |