| Full text | |
| Author(s): |
Zaninette, Fernanda
[1]
;
Lopes de Melo Rocha, Giulliana Aparecida
[1]
;
Bom Pessoni, Rosemeire Aparecida
[2]
;
Braga, Marcia Regina
[1]
;
Simoes, Kelly
[1]
;
Leone Figueiredo-Ribeiro, Rita de Cassia
[1]
;
Fialho, Mauricio Batista
[3]
Total Authors: 7
|
| Affiliation: | [1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, Sao Paulo - Brazil
[2] Univ Metodista Sao Paulo, Fac Saude, Sao Bernardo Do Campo - Brazil
[3] Univ Fed ABC, Prograd, Lab Biol, Ave Estados 5001, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Biotechnology and Applied Biochemistry; v. 66, n. 3, p. 419-425, MAY-JUN 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of beta-fructosyltransferases and/or beta-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in a medium supplemented with sucrose concentrations of 30, 100, or 150 g L-1. Hydrolytic activity on sucrose was observed in culture filtrates; however, at 150 g L-1 sucrose, the accumulation of 8 g L-1 1-kestose (inulin-type FOS) and 7.3 g L-1 neokestose (neolevan-type FOS) was observed, the latter being a type of FOS not commonly produced by filamentous fungi. In addition, minor amounts of four unidentified oligosaccharides, with a high degree of polymerization, were detected. The production of FOS was also observed in enzymatic assays, indicating the presence of extracellular enzymes with transfructosylating activity in the culture filtrates. Our findings demonstrate the feasibility of isolating promising microorganisms, for the production of FOS-synthesizing enzymes, from the rhizosphere of fructan-producing plants of the Brazilian Cerrado. (AU) | |
| FAPESP's process: | 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers |
| Grantee: | Marcia Regina Braga |
| Support Opportunities: | Research Projects - Thematic Grants |