Effects of intra-abdominal pressure in rat lung tissues after pneumoperitoneum

Autor(es)

Mendes Brandao, Julio Cezar ^[1] ; Dato, Carla Andria ^[1] ; Gaspar, Vanessa Coelho ^[1] ; Teixeira, Luciana Cristina ^[1] ; Masashi, Masashi Munechika ^[1] ; Ferez, David ^[1] ; dos Reis Falco, Luiz Fernando ^[1] ; Oliveira Junior, Itamar Souza ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Surg, Discipline Anesthesia Pain & Crit Care Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE; v. 12, n. 7, p. 8309-8317, 2019.
• Citações Web of Science: 0

Resumo

Background: Laparoscopic surgery requires pneumoperitoneum, achieved by pressure-controlled insufflation with carbon dioxide into the peritoneal cavity. This condition changes the respiratory metabolism, promoting lung damage. Purpose: The aim of the present study was to compare the effects of different levels of intra-abdominal pressure (IAP) in a ventilated rat model with normal lungs. Methods: Forty-eight Wistar rats were selected at random. Eight rats were assigned to each of the groups. The Sham group was subjected to a sham operation without pneumoperitoneum. The remaining groups were subjected to CO2 pneumoperitoneum with 5, 8, 10, 12, or 14 mmHg intra-abdominal pressure for 60 minutes. All animals were mechanically ventilated. At the end of the experiment, the animals were euthanatized. Their lungs were removed for analysis. Lipid peroxidation, myeloperoxidase activity, measurements of cytokines, and histopathological analysis were performed. Results: In the IAP5 group, all levels were lower, compared with those of other groups. TNF-alpha, IL-1beta, IL-6, lipid peroxidation, and myeloperoxidase activity were higher in groups IAP10, IAP12, and IAP14, compared with those of groups IAP5 and IAP8. Present results were supported by histopathological examinations. Conclusion: Present findings suggest that high-pressure increases oxidative stress and inflammatory-induced lung damage after pneumoperitoneum. (AU)

• Processo FAPESP: 14/12730-6 - Efeito do pneumoperitônio: estudo prospectivo experimental com análise comparativa de variações morfológicas e inflamatórias pulmonares
• Beneficiário: Itamar Souza de Oliveira-Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular