Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model

Autor(es):Mostrar menos -

Yanasse, Ricardo ^[1] ; De Labio, Roger ^[1] ; Marques, Leonardo ^[2] ; Fukasawa, Josianne ^[1] ; Segato, Rosimeire ^[1] ; Kinoshita, Angela ^[2] ; Matsumoto, Mariza ^[2] ; Felisbino, Sergio ^[3] ; Solano, Bruno ^[4] ; Dos Santos, Ricardo ^[4] ; Marques Payao, Spencer ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Fac Med Marilia FAMEMA, Blood Ctr, Dept Genet, Rua Lourival Freire 240, BR-17519050 Marilia, SP - Brazil

^[2] Univ Sagrado Coracao, Dept Hlth Sci, Bauru, SP - Brazil

^[3] Univ Estadual Paulista Julio de Mesquita Filho UN, BR-17519050 Botucatu, SP - Brazil

^[4] Monte Tabor Hosp Sao Rafael, Ctr Biotechnol & Cell Therapy, BR-17519050 Salvador, BA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EXPERIMENTAL AND THERAPEUTIC MEDICINE; v. 17, n. 6, p. 4344-4356, JUN 2019.
• Citações Web of Science: 0

Resumo

Stem cells in platelet-rich plasma (PRP) scaffolds may be a promising treatment for cartilage repair. Human dental pulp stem cell (hDPSC) subpopulations have been identified to have substantial angiogenic, neurogenic and regenerative potential when compared with other stem cell sources. The present study evaluated the potential of hDPSCs in a PRP scaffold to regenerate full-thickness cartilage defects in rabbits. Full-thickness articular cartilage defects were created in the patellar groove of the femur of 30 rabbits allocated into three experimental groups: Those with an untreated critical defect (CTL), those treated with PRP (PRP) and those treated with stem cells in a PRP scaffold (PRP+SC). The patellar grooves of the femurs from the experimental groups were evaluated macroscopically and histologically at 6 and 12 weeks post-surgery. The synovial membranes were also collected and evaluated for histopathological analysis. The synovial lining cell layer was enlarged in the CTL group compared with the PRP group at 6 weeks (P=0.037) but not with the PRP+SC group. All groups exhibited low-grade synovitis at 6 weeks and no synovitis at 12 weeks. Notably, macroscopic grades for the area of articular cartilage repair for the PRP+SC group were significantly improved compared with those in the CTL (P=0.001) and PRP (P=0.049) groups at 12 weeks. Furthermore, histological scores (modified O'Driscoll scoring system) of the patellar groove articular cartilage in the PRP+SC and PRP groups, in which the articular cartilage was primarily hyaline-like, were significantly higher compared with those in the CTL group at 12 weeks (P=0.002 and P=0.007, respectively). The present results support the therapeutic use of hDPSCs for the treatment of full-thickness articular cartilage defects. (AU)

• Processo FAPESP: 12/21007-0 - Utilização de células-tronco de polpa de dente decíduo humano em lesões de cartilagem articular de coelhos
• Beneficiário: Spencer Luiz Marques Payão
• Modalidade de apoio: Auxílio à Pesquisa - Regular