Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere

Autor(es)

Vargas Hoyos, Harold Alexander ^[1] ; Santos, Suikinai Nobre ^[1] ; Da Silva, Leonardo Jose ^[1] ; Paulino Silva, Fabio Sergio ^[1] ; Genuario, Diego Bonado ^[1] ; Zucchi, Tiago Domingues ^[2] ; Melo, Itamar Soares ^[1]

Afiliação do(s) autor(es)

^[1] Embrapa Environm, Lab Environm Microbiol, Rod SP 340, KM 127, 5, BR-13820000 Jaguariuna 1382000, SP - Brazil

^[2] Hinove Agrociencia, R Lilia Elisa Eberle Lupo 200B, BR-14803886 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY; v. 69, n. 8, p. 2431-2439, AUG 2019.
• Citações Web of Science: 0

Resumo

A novel actinobacterium, designated strain 1AS2c(T), was isolated from the wheat rhizosphere collected from the Brazilian Cerrado biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to Streptomyces cavourensis NBRC 13026(T), Streptomyces albolongus NBRC 13465(T) and Streptomyces araujoniae ASBV-1(T), sharing a similarity value of 98.2, 98.1 and 97.9 %, respectively. Additionally, MLSA of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA-DNA relatedness between strain 1AS2c(T) and its closest phylogenetic neighbours (S. cavourensis NBRC 13026(T), S. albolongus NBRC 13465 T and S. araujoniae ASBV-1(T): 56, 62.5 and 63.0 %, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2c(T) to the genus Streptomyces, representing a novel species. It is concluded that strain 1AS2c(T) (=CMAA 1679(T)=NRRL B-65479(T) =DSM 105299(T)) can be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces rhizosphaericola sp. nov. is proposed. (AU)

• Processo FAPESP: 13/16037-0 - Isolamento e caracterização de genes correlacionados a biossíntese de moléculas antitumorais em Streptomyces sp.
• Beneficiário: Suikinai Nobre Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/25076-0 - "Actinomicetos isolados de solos de ecossistemas brasileiros: potenciais agentes de controle biológico de Sclerotinia sclerotiorum em cultivo de soja (Glycine max L. Merrill)"
• Beneficiário: Harold Alexander Vargas Hoyos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/26131-7 - Análise do microbioma do Rio Amazonas e seus afluentes: biogeografia e sua contribuição na fixação do N2
• Beneficiário: Diego Bonaldo Genuário
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado