Monitoring indole alkaloid production by Penicillium digitatum during infection process in citrus by Mass Spectrometry Imaging and molecular networking

Autor(es)

Costa, Jonas Henrique ^[1] ; Bazioli, Jaqueline Moraes ^{[2, 1]} ; Araujo, Eder de Vilhena ^[1] ; Vendramini, Pedro Henrique ^[1] ; de Freitas Porto, Mariana Cristina C. ^[3] ; Eberlin, Marcos Nogueira ^[1] ; Souza-Neto, Jayme A. ^[3] ; Fill, Taicia Pacheco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Agr Sci, Dept Bioproc & Biotechnol, Cent Multiuser Lab, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 123, n. 8, p. 594-600, AUG 2019.
• Citações Web of Science: 2

Resumo

Green mold, caused by Penicillium digitatum, is the most destructive post-harvest disease in citrus. Secondary metabolites produced by fungal phytopathogens have been associated with toxicity to their respective host through the interaction with a wide range of cell targets. Natural products have also been described as important molecules for biocontrol and competition in their respective environment. For P. digitatum, the production of indole alkaloids, tryptoquialanines A and B, have been reported. However, their biological role remains unknown. Mass Spectrometry Imaging (MSI) technique was applied here for the first time to monitor the secondary metabolites produced on the orange surface during infection in order to gain insights about the P. digitatum-citrus interaction mechanisms. Through the combination of MSI and molecular networking it was possible to report, for the first time, the production of trypto-quivalines and fumiquinazolines by P. digitatum and also the accumulation of tryptoquialanines on the fruit surface from 4 to 7 d post inoculation. P. digitatum was also evaluated concerning the ability to sinthesize indole alkaloids in vivo in the different citrus hosts. The biological role of tryptoquialanines was investigated and tryptoquialanine A was submitted to insecticidal bioassays that revealed its high toxicity against Aedes Aegypti, suggesting an important insecticidal action during orange decay. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/11343-6 - Caracterização dos mecanismos de ação anti-dengue mediados pela microbiota intestinal de populações naturais do mosquito Aedes aegypti
• Beneficiário: Jayme Augusto de Souza-Neto
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/24462-4 - Entendendo as estratégias de virulência do fungo Penicillium digitam contra seu hospedeiro citros
• Beneficiário: Taicia Pacheco Fill
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/03670-0 - Prospecção de metabólitos secundários com atividade anticâncer produzidos por fungos fitopatogênicos
• Beneficiário: Jaqueline Moraes Bazioli
• Modalidade de apoio: Bolsas no Brasil - Mestrado