Monitoring indole alkaloid production by Penicillium digitatum during infection process in citrus by Mass Spectrometry Imaging and molecular networking

Author(s)

Costa, Jonas Henrique ^[1] ; Bazioli, Jaqueline Moraes ^{[2, 1]} ; Araujo, Eder de Vilhena ^[1] ; Vendramini, Pedro Henrique ^[1] ; de Freitas Porto, Mariana Cristina C. ^[3] ; Eberlin, Marcos Nogueira ^[1] ; Souza-Neto, Jayme A. ^[3] ; Fill, Taicia Pacheco ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083859 Campinas, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Agr Sci, Dept Bioproc & Biotechnol, Cent Multiuser Lab, Botucatu, SP - Brazil

• Document type: Journal article
• Source: FUNGAL BIOLOGY; v. 123, n. 8, p. 594-600, AUG 2019.
• Web of Science Citations: 2

Abstract

Green mold, caused by Penicillium digitatum, is the most destructive post-harvest disease in citrus. Secondary metabolites produced by fungal phytopathogens have been associated with toxicity to their respective host through the interaction with a wide range of cell targets. Natural products have also been described as important molecules for biocontrol and competition in their respective environment. For P. digitatum, the production of indole alkaloids, tryptoquialanines A and B, have been reported. However, their biological role remains unknown. Mass Spectrometry Imaging (MSI) technique was applied here for the first time to monitor the secondary metabolites produced on the orange surface during infection in order to gain insights about the P. digitatum-citrus interaction mechanisms. Through the combination of MSI and molecular networking it was possible to report, for the first time, the production of trypto-quivalines and fumiquinazolines by P. digitatum and also the accumulation of tryptoquialanines on the fruit surface from 4 to 7 d post inoculation. P. digitatum was also evaluated concerning the ability to sinthesize indole alkaloids in vivo in the different citrus hosts. The biological role of tryptoquialanines was investigated and tryptoquialanine A was submitted to insecticidal bioassays that revealed its high toxicity against Aedes Aegypti, suggesting an important insecticidal action during orange decay. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 13/11343-6 - Characterization of the microbiota-mediated anti-dengue mechanisms in wild Aedes aegypti populations
• Grantee: Jayme Augusto de Souza-Neto
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 17/24462-4 - Understanding the virulence strategies of Penicillium digitatum against the host citrus
• Grantee: Taicia Pacheco Fill
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/03670-0 - Prospecting for anticancer secondary metabolites produced by phytopathogenic fungi
• Grantee: Jaqueline Moraes Bazioli
• Support Opportunities: Scholarships in Brazil - Master