Biosynthetic studies involving bottom-up approaches of polyketides produced by Penicillium species

• Grant number: 15/10384-6
• Support Opportunities: Regular Research Grants
• Duration: February 01, 2016 - April 30, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Taicia Pacheco Fill
• Grantee: Taicia Pacheco Fill
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Biosynthesis of microbial natural products has aroused great interest in the scientific community and genetic studies of microorganisms already seem to represent the reality of modern research involving microbial biosynthesis of natural products. The fungus Penicillium brasilianum recently had its genome sequenced and the final assembly revealed 252 supercontigs which underwent gene predictions and automatic annotation. Functional genome analysis revealed 69 possible biosynthetic clusters, 11 clusters related to the biosynthesis of polyketides via PKS enzymes (polyketides synthase); 12 different clusters involved in the production of secondary metabolites formed via NRPS enzymes (non-ribosomal peptide synthetases) and 6 hybrids biosynthetic clusters, including the hybrid terpene-NRPS responsible for biosynthesis of alkaloids, indicating the great potential of this organism to produce bioactive secondary metabolites. The fungus Penicillium digitatum also is an interesting target for biosynthetic studies, mainly due to their pathogenicity against citrus, a serious problem for agriculture. Studies of their secondary metabolism and their respective biosynthesis would contribute greatly to the country. The fungus P. digitatum was recently sequenced and analysis of biosynthetic clusters of P. digitatum indicated 24 possible clusters, including 13 involved in the biosynthesis of peptides (NRPS enzymes), 14 possible clusters involved in the biosynthesis of polyketides via PKS enzymes and 3 clusters encoding the production of hybrid NRPS/PKS metabolites, indicating the potential of the targeted microorganisms. Thus, the project aims molecular biology studies and genetic studies of such fungi in the Organic Chemistry Department at the Chemistry Institute of UNICAMP, which converge to the possibility of biosynthetic studies of gene clusters of natural products found in Penicillium species, as well as enzyme studies using heterologous expression of biosynthetic enzymes of interest. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FILL, TAICIA PACHECO; BARETTA, JESSICA FERNANDA; PAULA DE ALMEIDA, LUIZ GONZAGA; MALAVAZI, IRAN; CERDEIRA, LOUISE TEIXEIRA; SAMBORSKYY, MARKIYAN; RIBEIRO DE VASCONCELOS, ANA TEREZA; LEADLAY, PETER; RODRIGUES-FILHO, EDSON. Draft Genome Sequence of the Fungus Penicillium brasilianum (Strain LaBioMMi 136), a Plant Endophyte from Melia azedarach. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 7, n. 21, NOV 2018. (10/11384-6, 15/10384-6)

BAZIOLI, JAQUELINE MORAES; AMARAL, LUCIANA DA SILVA; FILL, TAICIA PACHECO; RODRIGUES-FILHO, EDSON. Insights into Penicillium brasilianum Secondary Metabolism and Its Biotechnological Potential. Molecules, v. 22, n. 6, JUN 2017. (15/10384-6)

ARAUJO, EDER DE VILHENA; VENDRAMINI, PEDRO HENRIQUE; COSTA, JONAS HENRIQUE; EBERLIN, MARCOS NOGUEIRA; MONTAGNER, CASSIANA CAROLINA; FILL, TAICIA PACHECO. Determination of tryptoquialanines A and C produced by Penicillium digitatum in oranges: Are we safe?. Food Chemistry, v. 301, DEC 15 2019. (18/03670-0, 15/10384-6, 17/24462-4)

FILL, TAICIA PACHECO; PALLINI, HELOISA FASSINA; DIN, ZIA UD; JURBERG, IGOR DIAS; DA SILVA, JOSE VINICIUS; RODRIGUES-FILHO, EDSON. Conjugation of antifungal benzoic acid derivatives as a path for detoxification in Penicillium brasilianum, an endophyte from Melia azedarach. BIOORGANIC CHEMISTRY, v. 81, p. 367-372, DEC 2018. (10/11384-6, 15/10384-6)