Systemic administration of probiotics reduces the severity of apical periodontitis

Autor(es)

Cosme-Silva, L. ^[1] ; Dal-Fabbro, R. ^[1] ; Cintra, L. T. A. ^[1] ; dos Santos, V. R. ^[2] ; Duque, C. ^[2] ; Ervolino, E. ^[3] ; Mogami Bomfim, S. ^[4] ; Gomes-Filho, J. E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Endodont, Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Paediat & Social Dent, Aracatuba, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Aracatuba Vet Med, Dept Clin & Surg & Anim Reprod, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 52, n. 12 AUG 2019.
• Citações Web of Science: 0

Resumo

Aim To evaluate the effect of systemic administration of probiotics on the severity of apical periodontitis (AP). Methodology Twenty-four male Wistar rats were used. AP was induced in the maxillary left/right first molars. The animals were arranged into groups: Control, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Probiotics were administered orally for gavage (10(9) colony-forming units diluted in 5 mL of water for 30 days) during the development of AP. After 30 days, cardiac puncture was performed to analyse the complete blood count. Moreover, microbiological analysis of the root canal contents and saliva was performed. Then, the animals were euthanized and the jaw removed for histopathological and IL-10, IL-1 beta and IL-6 immunolabeling analyses. After the Shapiro-Wilk test of normality, the Kruskal-Wallis followed by Dunn's test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P < 0.05). Results No significance difference was observed in the blood profiles and in the counts of microorganisms from the saliva samples among the groups (P > 0.05). Total microorganism counts in the root canal, the inflammatory infiltrate and the immunostaining for IL-1 beta and IL-6 in AP were significantly lower in the probiotic groups when compared with the control group (P < 0.05). IL-10 was significantly more immunolabled in the probiotic groups than in the control group (P < 0.05). Conclusion Supplementation with probiotics (Lactobacillus rhamnosus and Lactobacillus acidophilus) had a significant effect on the severity of apical periodontitis in rats, demonstrating the anti-inflammatory effect of probiotics on the development of apical periodontitis. (AU)

• Processo FAPESP: 17/08312-2 - Efeito de probióticos no desenvolvimento de lesão periapical induzida em ratos
• Beneficiário: João Eduardo Gomes Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/08314-5 - Efeito de probióticos no desenvolvimento de lesão periapical induzida em ratos
• Beneficiário: Leopoldo Cosme Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado