Immunolocalisation and expression of oxytocin receptors and sex hormone-binding globulin in the testis and epididymis of dogs: correlation with sperm function

Autor(es):Mostrar menos -

Dalmazzo, Andressa ^[1] ; Losano, Joao D. A. ^[1] ; Angrimani, Daniel S. R. ^[1] ; Pereira, Isabel V. A. ^[2] ; Goissis, Marcelo D. ^[1] ; Francischini, Maria C. P. ^[1] ; Lopes, Everton ^[1] ; Minazaki, Claudia K. ^[3] ; Blank, Marcel H. ^[1] ; Cogliati, Bruno ^[2] ; Pereira, Ricardo J. G. ^[1] ; Barnabe, Valquiria H. ^[1] ; Nichi, Marcilio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Pathol, BR-05508270 Sao Paulo, SP - Brazil

^[3] Univ Paulista, BR-18087101 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 31, n. 9, p. 1434-1443, 2019.
• Citações Web of Science: 0

Resumo

The aim of this study was to confirm gene and protein expression of oxytocin receptor (OTR) and sex hormone-binding globulin (SHBG) in the testis and epididymis of dogs, correlating these data with sperm quality and production and testosterone concentrations. Positive correlations were found between OTR and SHBG expression in both the testis and epididymis. Testicular OTR expression was positively associated with plasma membrane and acrosome integrity in canine spermatozoa, whereas SHBG expression in the testis was positively correlated with various sperm characteristics, such as sperm concentration, total and progressive motility, plasma membrane integrity and acrosome integrity. Testicular expression of both OTR and SHBG was negatively correlated with low sperm mitochondrial activity. In the epididymis, SHBG expression was only positively correlated with plasma membrane integrity. Analysis of protein expression revealed that testicular OTR was positively correlated with testosterone concentrations and negatively correlated with the absence of sperm mitochondrial activity. In addition, SHBG expression in the testes was associated with epididymis SHBG expression and morphologically normal cells. Immunohistochemical (IHC) analysis revealed the presence of both OTR and SHBG in testicular smooth muscles and Leydig cells. However, in the epididymis, OTR was only located in smooth muscle cells, whereas neither IHC nor western blotting detected SHBG. Together, the results of this study suggest that OTR and SHBG play key roles in spermatogenesis and sperm maturation, being essential for male reproductive success. (AU)

• Processo FAPESP: 13/25674-4 - Correlação entre expressão do receptor de ocitocina (OTR) no testículo e epidídimo de cães com a qualidade espermática
• Beneficiário: Valquiria Hyppolito Barnabé
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/00888-1 - Correlação entre expressão do receptor de ocitocina (OTR) no testículo e epidídimo de cães com a qualidade espermática
• Beneficiário: Andressa Dalmazzo
• Modalidade de apoio: Bolsas no Brasil - Doutorado