Ginkgo biloba treatments reverse the impairment of conditioned suppression acquisition induced by GluN2B-NMDA and 5-HT1A receptor blockade: Modulatory effects of the circuitry of the dorsal hippocampal formation

Autor(es)

Zamberlam, Claudia R. ^{[1, 2]} ; Tilger, Myrcea A. S. ^[2] ; Moraes, Lais ^[1] ; Cerutti, Janete M. ^[1] ; Cerutti, Suzete M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Morfol & Genet, Lab Bases Genet Tumor Tiroide, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Ciencias Biol, Lab Farmacol Celular & Comportamental, Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Physiology & Behavior; v. 209, OCT 1 2019.
• Citações Web of Science: 0

Resumo

To improve our understanding of the effects of standardized extract of Ginkgo biloba (EGb) as a cognitive enhancer, we investigated the conditioned lick suppression induced expression (mRNA and protein) of the GluN2B-containing N-methyl-D-aspartic acid receptor (GluN2B-NMDAR), serotonin (5-HT) 1A receptor (5-HT1AR), gamma-aminobutyric acid type A receptor (GABA(A)R) and glial fibrillary acidic protein (GFAP) in the dorsal hippocampal formation (dHF) of untreated and EGb-treated (0.25, 0.5 and 1.0 g.kg(-1)) groups of rats. To substantiate our data, we analysed the molecular changes in dHF following treatment with vehicle, with agonists or antagonists of GABA(A)R, GluN2B-NMDAR and 5-HT1AR or with one of these antagonists prior to EGb and fear memory acquisition. Additionally, we performed a pharmacological analysis of the drug-receptor-receptor interactions and their supplemental role in fear memory by blocking individual receptors and analysed the possible changes in expression level with each of the other receptors in the study as well as astrocytes. Our data show for the first time that EGb treatment not only upregulated GluN2B, GABA(A)R-alpha 5, and GFAP compared with the control but also differentially upregulated GABA(A)R-alpha 1 in the dHF and 5HT(1A)R in the CA3. We found that the activation of GABA(A)Rs (diazepam) and the inactivation of GluN2B-NMDARs (Ro25-6981) or 5-HT1AR ((S)-WAY100135) resulted in memory impairment. Further, higher doses of EGb treatment reversed the effect of blocking GluN2B (P < 0.001) and 5-HT1AR (P < 0.001). Here, treatment with Ro25-6981 + EGb or (S)-WAY100135 + EGb prevented the impairment of the acquisition of lick suppression in association with the upregulation or prevention of the downregulation of Grin2b expression as well as the expression of GluN2B-NMDA and/or alpha 1 and alpha 5 subunit-containing GABA(A)R in the CA1 (P < 0.0001). Our data are in line with previous findings concerning the necessity of GluN2B for fear memory formation and add to the current knowledge of the role of the GABA(A)R-alpha 1 and -alpha 5 subunits and of GluN2B as a target of cognitive enhancers. Furthermore, our data show that these receptors play a complementary role in controlling the neural circuitry in the dHF that seems to be essential to conditioned lick suppression and the modulatory effects of EGb. (AU)

• Processo FAPESP: 13/20378-8 - Bases celulares e moleculares da aquisição e da extinção da supressão condicionada: prováveis alvos de ação do extrato padronizado de Ginkgo biloba
• Beneficiário: Suzete Maria Cerutti
• Modalidade de apoio: Auxílio à Pesquisa - Regular