Development, characterization and pre-clinical trials of an innovative wound healing dressing based on propolis (EPP-AF (R))-containing self-microemulsifying formulation incorporated in biocellulose membranes

Autor(es)

Marquele-Oliveira, Franciane ^[1] ; Barud, Hernane da Silva ^{[2, 3]} ; Torres, Elina Cassia ^{[1, 4]} ; Amaral Machado, Rachel Temperani ^[3] ; Caetano, Guilherme Ferreira ^[5] ; Leite, Marcel Nani ^[5] ; Cipriani Frade, Marco Andrey ^[5] ; Ribeiro, Sidney J. L. ^[3] ; Berretta, Andresa Aparecida ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Apis Flora Ind & Comercial Ltda, Lab Pesquisa Desenvolvimento & Inovacao P D&I, Rua Triunfo 945, BR-14020670 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Paulista Unesp, Inst Quim, CP 355, BR-14800900 Araraquara, SP - Brazil

^[3] Univ Araraquara Uniara, Lab Biopolimeros & Biomat BioPolMat, Araraquara, SP - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ave Cafe S-N, BR-14049900 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Div Dermatol, Dept Clin Med, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 136, p. 570-578, SEP 1 2019.
• Citações Web of Science: 1

Resumo

The considerable role of pristine bacterial cellulose membranes (BC) as ideal dressings have been widely demonstrated to treat wounds and burns. Nevertheless, drawbacks regarding antimicrobial spectrum and frequent dressing replacement are still present. Based on this, the present work proposes an innovative dressing by incorporating a technological self-microemulsifying formulation (SMEF) encapsulating propolis (BC/PP). BC/PP was fully chemically and biologically characterized employing in vitro and in vivo models. Antimicrobial studies demonstrated BC/PP high efficiency against both gran-negative and gran-positive bacteria. Release studies evidenced propolis markers sustained release for up to 7 days. In vivo wound healing activity was assessed by wound healing rate, anti-inflammatory and tissue formation events and the results evidenced the pro-inflammatory activity of BC/PP, which could promote improved healing results. To conclude, BC/PP presented an outstanding antibacterial activity in vitro with weekly replacement and promotion of healing, offering, for the first time, a broadspectrum biomembrane potential to treat infected wounds. (C) 2019 Published by Elsevier B.V. (AU)

• Processo FAPESP: 11/51725-0 - Desenvolvimento e avaliação de biocurativos obtidos a partir de celulose bacteriana e extrato padronizado de Própolis (EPP-AF) para o tratamento de queimaduras e/ou lesões de pele
• Beneficiário: Hernane da Silva Barud
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE