Is breath testing without C-13-labelled external urea able to detect Helicobacter pylori infection?

Autor(es)

Marques Carlos, Ana Beatriz ^[1] ; de Oliveira, Cassio Vieira ^[2] ; Marchesan Rodrigues, Maria Aparecida ^[3] ; Costa, Vladimir Eliodoro ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Inst Biosci IBB, Stable Isotope Ctr, BR-18618689 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Div Gastroenterol, Dept Internal Med, BR-18618687 Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Dept Pathol, BR-18618687 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 411, n. 22, p. 5641-5645, SEP 2019.
• Citações Web of Science: 0

Resumo

Helicobacter pylori (H. pylori) infection is the main cause of gastric inflammation and peptic ulcer disease. Diagnosis and treatment are important to prevent these outcomes. The diagnosis of H. pylori infection can be performed by non-invasive methods, such as C-13-urea breath test (C-13-UBT). As endogenous urea is normally released to body cavities, we sought to investigate the usefulness of UBT without C-13-labelled external urea to detect H. pylori infection. The analysis was performed in a series of adult patients just before upper gastrointestinal endoscopy and biopsy to investigate dyspeptic symptoms. Breath samples were analyzed using isotope ratio mass spectrometry (IRMS). The natural variation of C-13 and O-18 isotopic abundance in the breath samples was also investigated. The results of the isotopic analysis were compared with the findings of the histopathological evaluation of gastric biopsies, which is the gold standard to detect H. pylori infection. No differences between patients with or without H. pylori infection could be detected by the isotope analysis of breath tests without C-13-urea. Therefore, our results showed that UBT without C-13-urea, analyzed by IRMS, was not useful to detect H. pylori infection in the study population. (AU)

• Processo FAPESP: 17/13021-7 - Teste respiratório com uréia endógena e analisado por IRMS para detecção da infecção pelo Helicobacter pylori
• Beneficiário: Ana Beatriz Marques Carlos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica