Induced pluripotent stem cells throughout the animal kingdom: Availability and applications

Autor(es)

Pessoa, Lais Vicari de Figueiredo ^[1] ; Bressan, Fabiana Fernandes ^[2] ; Freude, Kristine Karla ^[1]

Afiliação do(s) autor(es)

^[1] Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet & Anim Sci, Grp Stem Cell Models Studies Neurodegenerat Dis, S, Gronnegardsvej 7, DK-1870 Frederiksberg - Denmark

^[2] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635000 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: WORLD JOURNAL OF STEM CELLS; v. 11, n. 8, p. 491-505, AUG 26 2019.
• Citações Web of Science: 1

Resumo

Up until the mid 2000s, the capacity to generate every cell of an organism was exclusive to embryonic stem cells. In 2006, researchers Takahashi and Yamanaka developed an alternative method of generating embryonic-like stem cells from adult cells, which they coined induced pluripotent stem cells (iPSCs). Such iPSCs possess most of the advantages of embryonic stem cells without the ethical stigma associated with derivation of the latter. The possibility of generating ``custom-made{''} pluripotent cells, ideal for patient-specific disease models, alongside their possible applications in regenerative medicine and reproduction, has drawn a lot of attention to the field with numbers of iPSC studies published growing exponentially. IPSCs have now been generated for a wide variety of species, including but not limited to, mouse, human, primate, wild felines, bovines, equines, birds and rodents, some of which still lack well-established embryonic stem cell lines. The paucity of robust characterization of some of these iPSC lines as well as the residual expression of transgenes involved in the reprogramming process still hampers the use of such cells in species preservation or medical research, underscoring the requirement for further investigations. Here, we provide an extensive overview of iPSC generated from a broad range of animal species including their potential applications and limitations. (AU)

• Processo FAPESP: 15/26818-5 - Investigação de mecanismos celulares e moleculares da aquisição da toti- e pluripotência induzida in vitro - modelo translacional
• Beneficiário: Fabiana Fernandes Bressan
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores