Optimizing the potential bioactivity of isoflavones from soybeans via ultrasound pretreatment: Antioxidant potential and NF-kappa B activation

Autor(es)

Falcao, Heloisa Gabriel ^[1] ; Ramos Silva, Mariah Benine ^[1] ; de Camargo, Adriano Costa ^{[2, 1]} ; Shahidi, Fereidoon ^[2] ; Franchin, Marcelo ^[3] ; Rosalen, Pedro Luiz ^[3] ; Alencar, Severino Matias ^[4] ; Kurozawa, Louise Emy ^[5] ; Ida, Elza Iouko ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Dept Food Sci & Technol, BR-86051990 Londrina, Parana - Brazil

^[2] Mem Univ Newfoundland, Dept Biochem, St John, NF - Canada

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba - Brazil

^[4] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agri Food Ind, Food & Nutr, Piracicaba - Brazil

^[5] Univ Estadual Campinas, Fac Food Engn, Dept Food Engn, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FOOD BIOCHEMISTRY; v. 43, n. 11 AUG 2019.
• Citações Web of Science: 2

Resumo

Soybean consumption has been associated with health benefits. However, the effect of ultrasound (US) soybean pretreatment in terms of potential health benefits has not been investigated so far. Accordingly, the total phenolic content (TPC) and the total aglycone content (TAC) were optimized using the Box-Behnken design. Contrasting samples regarding isoflavones aglycones and TPCs were screened for their antioxidant and anti-inflammatory potentials using RAW 264.7 macrophages. US pretreated soybeans (55 degrees C, 15 min, and 24 W/cm(2)) showed greater TPC and TAC compared to the control and this translated to higher antiradical activity and reduction of nuclear factor kappa B (NF-kappa B) activation. The concentration of genistein in treated soybeans increased by 95%. Furthermore, US pretreated soybeans rendered phenolic extracts that reduced the NF-kappa B activation by 86%. Therefore, this contribution demonstrates the beneficial effects of US pretreatment of soybeans, which provides a better feedstock for the functional food industry. Practical applications Soybeans can be consumed as such or used as a feedstock to produce soy yogurt, fermented soymilk, tofu, and protein concentrate, among others. The greatest bioavailability of isoflavones compared to other flavonoids has recently been highlighted, and this has been explained by the relatively moderate lipophilicity of isoflavones as aglycones. The present contribution supports the use of US pretreatment of soybeans to obtain a feedstock with improved contents of isoflavones as aglycones. We have confirmed that phenolic extracts obtained from the US pretreated samples showed higher bioactivity as radical scavengers and by reducing the activation of nuclear factor kappa B (NF-kappa B) in a cell model, which is mediated by oxidative species. The clinical importance of NF-kappa B activation is derived mainly from its role in inflammatory responses. Therefore, our investigation may have a practical application in the procurement of soybean products and/or ingredients with improved functional properties related to their health benefits. (AU)

• Processo FAPESP: 16/15563-9 - Caracterização de novos alvos farmacológicos e estudo da atividade de uma 4-fenil cumarina na diferenciação de linfócitos Th17 no desenvolvimento da doença periodontal
• Beneficiário: Marcelo Franchin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/09898-0 - Bioprospecção de novas moléculas anti-inflamatórias de produtos naturais nativos brasileiros
• Beneficiário: Pedro Luiz Rosalen
• Modalidade de apoio: Auxílio à Pesquisa - Regular