Autor(es)

Scarpellini, Carolina da Silveira ^{[1, 2, 3]} ; Cristina-Silva, Caroline ^{[1, 2]} ; Biancardi, Vivian ^{[1, 2, 4]} ; Gargaglioni, Luciane H. ^[1] ; Almeida, Maria Camila ^[5] ; Bicego, Kenia Cardoso ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Coll Agr & Vet Sci, Dept Anim Morphol & Physiol, Via Acesso Prof Paulo Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Joint UFSCar UNESP Grad Program Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil

^[3] St Michaels Hosp, Keenan Res Ctr Biomed Sci, Toronto, ON M5B 1T8 - Canada

^[4] Univ Alberta, Fac Med & Dent, Dept Physiol, Edmonton, AB T6G 2R7 - Canada

^[5] Univ Fed ABC UFABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY; v. 471, n. 9, p. 1191-1203, SEP 2019.
• Citações Web of Science: 0

Resumo

Recently, we have described, in non-genetically modified rats, that peripheral transient receptor potential vanilloid-4 (TRPV4) channels are activated and trigger warmth-defence responses at ambient temperatures of 26-30 degrees C. Evidence points to the presence of TRPV4 in the medial preoptic area, a region described to be involved in the activation of thermoeffector pathways, including those involved in heat loss. Thus, we tested the hypothesis that TRPV4 in the medial preoptic area modulates thermoregulation under warm conditions. To this end, under two ambient temperatures (21 and 28 degrees C), body temperature was measured in rats following blockade of preoptic TRPV4 with two antagonists, HC-067047 and GSK 2193874. Oxygen consumption, heat loss index and preferred ambient temperature were also determined in order to assess thermoeffector activity. Antagonism of central TRPV4 caused an increase in body temperature in rats exposed to 28 degrees C, but not in those exposed to 21 degrees C. The body temperature increase at 28 degrees C was accompanied by an increase in oxygen consumption and an earlier reduction of the heat loss index. In behavioural experiments, control animals previously exposed to warm ambient temperatures (28-30 degrees C) for 2 h selected colder temperatures in a thermogradient compared to those injected with HC-067047. Our results support the idea that preoptic TRPV4 modulates thermoregulation in a warm environment by activating both autonomic and behavioural heat loss responses. Thus, according to the present study and to that published recently by our group, the activation of warmth-defence responses by TRPV4 seems to be dependent on the activity of both peripheral and central channels. (AU)

• Processo FAPESP: 12/15298-2 - Padrão cardiorrespiratório e ciclo sono-vigília de ratos e ratas expostos à fluoxetina durante o período pré-natal
• Beneficiário: Vivian Biancardi Rossato
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/04849-6 - Participação dos canais TRPV4 na termorregulação de endotermos
• Beneficiário: Kênia Cardoso Bícego
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/19131-2 - Participação dos canais TRPV4 hipotalâmicos na ativação/inibição dos termoefetores autonômicos durante a febre e o choque endotoxêmico
• Beneficiário: Carolina da Silveira Scarpellini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/17278-2 - Participação da rafe no controle de termoefetores em ave precoce
• Beneficiário: Caroline Cristina Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/02991-0 - Envolvimento dos canais TRPM8 na termorregulação de ratos Wistar
• Beneficiário: Maria Camila Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular