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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hypothalamic TRPV4 channels participate in the medial preoptic activation of warmth-defence responses in Wistar male rats

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Scarpellini, Carolina da Silveira [1, 2, 3] ; Cristina-Silva, Caroline [1, 2] ; Biancardi, Vivian [1, 2, 4] ; Gargaglioni, Luciane H. [1] ; Almeida, Maria Camila [5] ; Bicego, Kenia Cardoso [1]
Total Authors: 6
[1] Sao Paulo State Univ, Coll Agr & Vet Sci, Dept Anim Morphol & Physiol, Via Acesso Prof Paulo Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil
[2] Joint UFSCar UNESP Grad Program Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil
[3] St Michaels Hosp, Keenan Res Ctr Biomed Sci, Toronto, ON M5B 1T8 - Canada
[4] Univ Alberta, Fac Med & Dent, Dept Physiol, Edmonton, AB T6G 2R7 - Canada
[5] Univ Fed ABC UFABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY; v. 471, n. 9, p. 1191-1203, SEP 2019.
Web of Science Citations: 0

Recently, we have described, in non-genetically modified rats, that peripheral transient receptor potential vanilloid-4 (TRPV4) channels are activated and trigger warmth-defence responses at ambient temperatures of 26-30 degrees C. Evidence points to the presence of TRPV4 in the medial preoptic area, a region described to be involved in the activation of thermoeffector pathways, including those involved in heat loss. Thus, we tested the hypothesis that TRPV4 in the medial preoptic area modulates thermoregulation under warm conditions. To this end, under two ambient temperatures (21 and 28 degrees C), body temperature was measured in rats following blockade of preoptic TRPV4 with two antagonists, HC-067047 and GSK 2193874. Oxygen consumption, heat loss index and preferred ambient temperature were also determined in order to assess thermoeffector activity. Antagonism of central TRPV4 caused an increase in body temperature in rats exposed to 28 degrees C, but not in those exposed to 21 degrees C. The body temperature increase at 28 degrees C was accompanied by an increase in oxygen consumption and an earlier reduction of the heat loss index. In behavioural experiments, control animals previously exposed to warm ambient temperatures (28-30 degrees C) for 2 h selected colder temperatures in a thermogradient compared to those injected with HC-067047. Our results support the idea that preoptic TRPV4 modulates thermoregulation in a warm environment by activating both autonomic and behavioural heat loss responses. Thus, according to the present study and to that published recently by our group, the activation of warmth-defence responses by TRPV4 seems to be dependent on the activity of both peripheral and central channels. (AU)

FAPESP's process: 12/15298-2 - Cardiorespiratory pattern and awake-sleep state in male and female rats exposed to fluoxetine during prenatal period
Grantee:Vivian Biancardi Rossato
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/04849-6 - Role of TRPV4 channels in thermoregulation in endotherms
Grantee:Kênia Cardoso Bícego
Support type: Regular Research Grants
FAPESP's process: 11/19131-2 - Role of hypothalamic TRPV4 channels in activation/inhibition of autonomic thermoeffectors during fever and endotoxemic chock
Grantee:Carolina da Silveira Scarpellini
Support type: Scholarships in Brazil - Doctorate
Grantee:Caroline Cristina Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/02991-0 - Involvement of TRPM8 channels in thermoregulation of Wistar rats
Grantee:Maria Camila Almeida
Support type: Regular Research Grants