Prevalence of Ehrlichia canis (Rickettsiales: Ehrlichieae) DNA in Tissues From Rhipicephalus sanguineus (Acari: Ixodidae) Ticks in Areas Endemic for Canine Monocytic Ehrlichiosis in Brazil

Autor(es)

Miranda Oliveira, Bruno Cesar ^[1] ; Ferrari, Elis Domingos ^[1] ; Viol, Milena Arauz ^[1] ; Andre, Marcos Rogerio ^[2] ; Machado, Rosangela Zacarias ^[2] ; Costa de Aquino, Monally Conceicao ^[1] ; Inacio, Sandra Valeria ^[1] ; Gomes, Jancarlo Ferreira ^[3] ; Guerrero, Felix D. ^[4] ; Saraiva Bresciani, Katia Denise ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Unesp, Fac Med Vet Aracatuba, Clovis Pestana St 793, BR-16050680 Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Ciencias Agrarias & Vet Jaboticabal, Via Acesso Prof Paulo Donato Castelane, Castellane, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Campinas UNICAMP, Cidade Univ Zeferino Vaz, BR-13083970 Campinas, SP - Brazil

^[4] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700, Fredericksburg Rd, Kerrville, TX 78028 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Entomology; v. 56, n. 3, p. 828-831, MAY 2019.
• Citações Web of Science: 1

Resumo

Canine monocytic ehrlichiosis (CME) is a disease caused by the obligate intracellular bacterium Ehrlichia canis. Tropical lineages of Rhipicephalus sanguineus ticks play an essential role in the transmission of this pathogen. The aim of the present study was to evaluate the prevalence of E. canis DNA in tissue from R. sanguineus ticks in areas endemic for CME in Brazil and quantify levels of E. canis DNA in dissected tissues from these samples. A total of 720 ticks were collected from 72 dogs (36 dogs from the city Aracatuba in Sao Paulo state and 36 from Campo Grande in the state of Mato Grosso do Sul). Ticks were dissected to collect the guts, ovaries and salivary gland. A quantitative polymerase chain reaction (qPCR) targeting the disulphide bond formation (dsb) protein gene was performed to quantify the level of E. canis infection. The E. canis dsb-qPCR assay was positive for 31.9, 10, and 15.2% of the gut, ovary, and salivary glands, respectively. The average gut, ovary, and salivary gland bacterial load estimated by qPCR was 1.21 x 10(3), 2.60 x 10(3), and 4.92 x 10(3) gene copies/mu l, respectively. This is the first report of E. canis DNA in ovaries of R. sanguineus ticks parasitizing dogs in these CME-endemic areas. These observations raise the possibility of E. canis trans-ovarial transmission. (AU)

• Processo FAPESP: 14/26461-7 - Detecção molecular e sorológica de Ehrlichia canis em cães e em órgãos de seus respectivos carrapatos em duas regiões endêmicas
• Beneficiário: Bruno César Miranda Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado