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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of Zirconia-Coated Bioactive Glass on Gingival Fibroblast Behavior

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Autor(es):
Suelen Aline de Lima Barros [1] ; Diana Gabriela Soares [2] ; Maria Luísa Leite [3] ; Fernanda Gonçalves Basso [4] ; Carlos Alberto de Souza Costa [5] ; Gelson Luís Adabo [6]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
[2] Universidade de São Paulo. Endodontics and Dental Materials. Department of Operative Dentistry - Brasil
[3] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
[4] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[5] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[6] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Brazilian Dental Journal; v. 30, n. 4, p. 333-341, 2019-07-22.
Resumo

Abstract The objective of this study was the development of a bioactive glass coating on zirconia (Zr) to modulate the gingival fibroblast phenotype. For this purpose, Biosilicate® (BS) particles in a water/isopropyl alcohol (1:1) vehicle (6 mg/mL) were applied to zirconia discs followed by thermal treatment at 1100 °C for 20 min. The surface topography (SEM), chemical composition (EDX), surface roughness (Ra; confocal microscopy), surface free energy (goniometry), and color alteration (UV-vis spectrophotometry) were assessed (n=6). Thereafter, L929 fibroblasts were seeded onto Zr and Zr+BS discs, and cell proliferation (Alamar Blue; n=6), morphology (SEM; n=2), migration (wound healing; n=4), and collagen synthesis (Sirius Red; n=6) were evaluated up to 7 days. Data were analyzed by ANOVA/Tukey tests (a=5%). A homogeneous coating consisting of Si, Na, O, and Ca was detected on the Zr surface after thermal treatment with BS, which led to a significant increase in surface roughness and free energy (p<0.05). No change in color parameters was observed (p>0.05). Cells seeded on the Zr+BS surface featured increased proliferation, collagen expression, and migration capability in comparison with those cultured on plain Zr (p<0.05). SEM images revealed that cell spreading occurred faster in the presence of BS. Therefore, it was concluded that thermal treatment of the Zr surface with BS led to the deposition of a bioactive coating, which induced gingival fibroblast spread, proliferation, migration, and collagen expression in vitro. (AU)

Processo FAPESP: 15/19034-8 - Efeitos da incorporação de um biovidro sobre superfície da zircônia: avaliação de propriedades físicas, químicas e biológicas
Beneficiário:Suelen Aline de Lima Barros
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/15674-5 - Associação de técnicas de engenharia tecidual para modulação da regeneração de tecidos mineralizados sob inflamação degenerativa: análise em modelos de cultura-3D em biorreator de perfusão e inflamatórios em animais
Beneficiário:Diana Gabriela Soares dos Passos
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores