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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of Zirconia-Coated Bioactive Glass on Gingival Fibroblast Behavior

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Author(s):
Suelen Aline de Lima Barros [1] ; Diana Gabriela Soares [2] ; Maria Luísa Leite [3] ; Fernanda Gonçalves Basso [4] ; Carlos Alberto de Souza Costa [5] ; Gelson Luís Adabo [6]
Total Authors: 6
Affiliation:
[1] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
[2] Universidade de São Paulo. Endodontics and Dental Materials. Department of Operative Dentistry - Brasil
[3] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
[4] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[5] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[6] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Dental Journal; v. 30, n. 4, p. 333-341, 2019-07-22.
Abstract

Abstract The objective of this study was the development of a bioactive glass coating on zirconia (Zr) to modulate the gingival fibroblast phenotype. For this purpose, Biosilicate® (BS) particles in a water/isopropyl alcohol (1:1) vehicle (6 mg/mL) were applied to zirconia discs followed by thermal treatment at 1100 °C for 20 min. The surface topography (SEM), chemical composition (EDX), surface roughness (Ra; confocal microscopy), surface free energy (goniometry), and color alteration (UV-vis spectrophotometry) were assessed (n=6). Thereafter, L929 fibroblasts were seeded onto Zr and Zr+BS discs, and cell proliferation (Alamar Blue; n=6), morphology (SEM; n=2), migration (wound healing; n=4), and collagen synthesis (Sirius Red; n=6) were evaluated up to 7 days. Data were analyzed by ANOVA/Tukey tests (a=5%). A homogeneous coating consisting of Si, Na, O, and Ca was detected on the Zr surface after thermal treatment with BS, which led to a significant increase in surface roughness and free energy (p<0.05). No change in color parameters was observed (p>0.05). Cells seeded on the Zr+BS surface featured increased proliferation, collagen expression, and migration capability in comparison with those cultured on plain Zr (p<0.05). SEM images revealed that cell spreading occurred faster in the presence of BS. Therefore, it was concluded that thermal treatment of the Zr surface with BS led to the deposition of a bioactive coating, which induced gingival fibroblast spread, proliferation, migration, and collagen expression in vitro. (AU)

FAPESP's process: 15/19034-8 - Effects of incorporation of a bioglass on zirconia surface: evaluation of physical, chemical and biological proprieties
Grantee:Suelen Aline de Lima Barros
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/15674-5 - Association of tissue engineering techniques for mineralized tissue regeneration under degenerative inflammatory stimulus: analysis on 3D-culture perfusion bioreactor and animal inflammatory models
Grantee:Diana Gabriela Soares dos Passos
Support Opportunities: Research Grants - Young Investigators Grants