Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

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da Silva, Helio Rodrigues ^[1] ; Mamani, Javier Bustamante ^[1] ; Nucci, Mariana Penteado ^[2] ; Nucci, Leopoldo Penteado ^[3] ; Kondo, Andrea Tiemi ^[1] ; Carvalho Fantacini, Daianne Maciely ^[4] ; Botelho de Souza, Lucas Eduardo ^[4] ; Picanco-Castro, Virginia ^[4] ; Covas, Dimas Tadeu ^[4] ; Kutner, Jose Mauro ^[1] ; de Oliveira, Fernando Anselmo ^[1] ; Hamerschlak, Nelson ^[1] ; Gamarra, Lionel Fernel ^[1]

Afiliação do(s) autor(es)

^[1] Hosp Israelita Albert Einstein, BR-05651900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, LIM44, Hosp Clin HCFMUSP, Fac Med, BR-05403010 Sao Paulo - Brazil

^[3] Ctr Univ Planalto Cent, BR-72445020 Brasilia, DF - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-05403010 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF STEM CELLS; v. 11, n. 2, p. 100-123, FEB 26 2019.
• Citações Web of Science: 0

Resumo

BACKGROUND Mesenchymal stem cells(MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles (MNPs) using new labels and covers to achieve low toxicity and longtime residence in cells. AIM To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke model. METHODS After the isolation and immunophenotypic characterization of human bone marrow MSCs (hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images (BLIs) in vitro and in vivo. In addition, MNPs that were previously characterized (regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells, analyze cell viability via the 3-{[}4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging (MRI), near-infrared fluorescence (NIRF), and inductively coupled plasma-mass spectrometry (ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations (after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal images. RESULTS hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with luciferase (hBM-MSC Luc), we detected a maximum BLI intensity of 2.0 x 108 photons/s in samples of 106 hBM-MSCs. Analysis of the physicochemical characteristics of the MNPs showed an average hydrodynamic diameter of 38.2 +/- 0.5 nm, zeta potential of 29.2 +/- 1.9 mV and adequate colloidal stability without agglomeration over 18 h. The signal of iron load internalization in hBM-MSC Luc showed a close relationship with the corresponding MNP-labeling concentrations based on MRI, ICP-MS and NIRF. Under the highest MNP concentration, cellular viability showed a reduction of less than 10% compared to the control. Correlation analysis of the MNP load internalized into hBM-MSCLuc determined via the MRI, ICP-MS and NIRF techniques showed the same correlation coefficient of 0.99. Evaluation of the BLI, NIRF, and MRI signals in vivo and ex vivo after labeled hBM-MSCLuc were implanted into animals showed differences between different MNP concentrations and signals associated with different techniques (MRI and NIRF; 5 and 20 mu g Fe/mL; P < 0.05) in the sham groups at 4 h as well as a time effect (4 h and 6 d; P < 0.001) and differences between the sham and stroke groups in all images signals (P < 0.001). CONCLUSION This study highlighted the importance of quantifying MNPs internalized into cells and the efficacy of signal detection under the triple-image modality in a stroke model. (AU)

• Processo FAPESP: 14/50983-3 - INCT 2014: fluidos complexos
• Beneficiário: Antonio Martins Figueiredo Neto
• Linha de fomento: Auxílio à Pesquisa - Temático