Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Image and motor behavior for monitoring tumor growth in C6 glioma model

Texto completo
Autor(es):
Mostrar menos -
Felix Souza, Taylla Klei [1] ; Nucci, Mariana Penteado [2] ; Mamani, Javier Bustamante [1] ; da Silva, Helio Rodrigues [1] ; Carvalho Fantacini, Daianne Maciely [3] ; Botelho de Souza, Lucas Eduardo [3] ; Picanco-Castro, Virginia [3] ; Covas, Dimas Tadeu [3] ; Vidoto, Edson Luis [4] ; Tannus, Alberto [4] ; Gamarra, Lionel Fernel [5, 1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, LIM44, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Inst Fis Sao Carlos, CIERMag, Sao Paulo - Brazil
[5] Santa Casa Misericordia Sao Paulo, Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 13, n. 7 JUL 26 2018.
Citações Web of Science: 3
Resumo

The primary objective of this study is to monitor tumor growth by using image techniques and behavioral testing through general and specific motor activities (spontaneous movements and gait). Our sample includes male Wistar rats, 2 months old and weighing 250-300 g, that is categorized into three groups: control, sham, and experimental. The experimental group was anesthetized; the C6 cells with luciferase expression that were suspended in a culture medium were implanted into the right frontoparietal cortex of the rats. The sham group received implant only with culture medium without cells. Images and behavioral tests were evaluated at base time and at 7, 14, 21, and 28 days after induced tumor growth analysis. The tumor volume measured by magnetic resonance imaging (MRI) and quantitative bioluminescence imaging (BLI) signal showed a correlation coefficient of r = 0.96. The MRI showed that the mean tumor volume increased by approximately 10, 26, and 49 times according to a comparison of tumor volume on the seventh day with 14, 21, and 28 days, respectively. The quantification of the BLI signal was (4.12 +/- 2.01) x 10(8), (8.33 +/- 3.12) x 10(8), (28.43 +/- 6.32) x 10(8), and (63.02 +/- 10.53) x 10(8) photons/s at the seventh, fourteenth, twenty-first, and twenty-eighth day, respectively. After 14 days of tumor induction, both behavioral tests showed significant differences between tumor and sham or control groups. Our study showed a high correlation between MRI and BLI for tumor growth monitoring with complement aspects analysis in tumor volume. In addition, functional behavioral analysis displayed sensitivity to monitor tumor growth, as well as to detect early significant changes between groups, primarily in the tumor group. The results of gait analysis were more sensitive than general motor analysis. (AU)

Processo FAPESP: 16/21470-3 - Ação terapêutica das células tronco mesenquimais da medula humana, marcadas com nanopartículas multimodais em ratos diabéticos submetidos a isquemia cerebral focal: estudo dos mecanismos celulares, moleculares e funcionais
Beneficiário:Lionel Fernel Gamarra Contreras
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50983-3 - INCT 2014: fluidos complexos
Beneficiário:Antonio Martins Figueiredo Neto
Linha de fomento: Auxílio à Pesquisa - Temático