| Full text | |
| Author(s): Show less - |
Felix Souza, Taylla Klei
[1]
;
Nucci, Mariana Penteado
[2]
;
Mamani, Javier Bustamante
[1]
;
da Silva, Helio Rodrigues
[1]
;
Carvalho Fantacini, Daianne Maciely
[3]
;
Botelho de Souza, Lucas Eduardo
[3]
;
Picanco-Castro, Virginia
[3]
;
Covas, Dimas Tadeu
[3]
;
Vidoto, Edson Luis
[4]
;
Tannus, Alberto
[4]
;
Gamarra, Lionel Fernel
[5, 1]
Total Authors: 11
|
| Affiliation: | [1] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Med, LIM44, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Inst Fis Sao Carlos, CIERMag, Sao Paulo - Brazil
[5] Santa Casa Misericordia Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | PLoS One; v. 13, n. 7 JUL 26 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
The primary objective of this study is to monitor tumor growth by using image techniques and behavioral testing through general and specific motor activities (spontaneous movements and gait). Our sample includes male Wistar rats, 2 months old and weighing 250-300 g, that is categorized into three groups: control, sham, and experimental. The experimental group was anesthetized; the C6 cells with luciferase expression that were suspended in a culture medium were implanted into the right frontoparietal cortex of the rats. The sham group received implant only with culture medium without cells. Images and behavioral tests were evaluated at base time and at 7, 14, 21, and 28 days after induced tumor growth analysis. The tumor volume measured by magnetic resonance imaging (MRI) and quantitative bioluminescence imaging (BLI) signal showed a correlation coefficient of r = 0.96. The MRI showed that the mean tumor volume increased by approximately 10, 26, and 49 times according to a comparison of tumor volume on the seventh day with 14, 21, and 28 days, respectively. The quantification of the BLI signal was (4.12 +/- 2.01) x 10(8), (8.33 +/- 3.12) x 10(8), (28.43 +/- 6.32) x 10(8), and (63.02 +/- 10.53) x 10(8) photons/s at the seventh, fourteenth, twenty-first, and twenty-eighth day, respectively. After 14 days of tumor induction, both behavioral tests showed significant differences between tumor and sham or control groups. Our study showed a high correlation between MRI and BLI for tumor growth monitoring with complement aspects analysis in tumor volume. In addition, functional behavioral analysis displayed sensitivity to monitor tumor growth, as well as to detect early significant changes between groups, primarily in the tumor group. The results of gait analysis were more sensitive than general motor analysis. (AU) | |
| FAPESP's process: | 16/21470-3 - Therapeutic action of mesenchymal stem cells from human bone marrow labeled with multimodal nanoparticles in diabetic rats subjected to stroke: study of cellular, molecular and functional mechanisms. |
| Grantee: | Lionel Fernel Gamarra Contreras |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50983-3 - INCT 2014: complex fluids |
| Grantee: | Antonio Martins Figueiredo Neto |
| Support Opportunities: | Research Projects - Thematic Grants |