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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photobiomodulation combined with photodynamic therapy using ruthenium phthalocyanine complexes in A375 melanoma cells: Effects of nitric oxide generation and ATP production

Texto completo
Autor(es):
Negri, Laisa Bonafim [1] ; Martins, Tassia Joi [2] ; da Silva, Roberto Santana [3, 2, 1] ; Hamblin, Michael R. [3, 4]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ave Cafe S-N, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil
[3] Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 - USA
[4] Harvard Med Sch, Dept Dermatol, Boston, MA 02115 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 198, SEP 2019.
Citações Web of Science: 1
Resumo

Light irradiation has been used in clinical therapy for several decades. In this context, photobiomodulation (PBM) modulates signaling pathways via ROS, ATP, Ca2+, while photodynamic therapy (PDT) generates reactive oxygen species by excitation of a photosensitizer. NO generation could be an important tool when combined with both kinds of light therapy. By using a metal-based compound, we found that PBM combined with PDT could be a beneficial cancer treatment option. We used two types of ruthenium compounds, ({[}Ru(Pc)], Pc = phthalocyanine) and trans-{[}Ru(NO)(NO2)(Pc)]. The UV-vis spectra of both complexes displayed a band in the 660 nm region. In the case of 0.5 mu M trans-{[}Ru(NO)(NO2)(Pc)], light irradiation at the Q-band reduced the percentage of viable human melanoma (A375) cells to around 50% as compared to {[}Ru(Pc)]. We hypothesized that these results were due to a synergistic effect between singlet oxygen and nitric oxide. Similar experiments performed with PDT (660 nm) combined with PBM (850 nm) induced more photocytotoxicity using both {[}Ru (Pc)] and trans-{[}Ru(NO)(NO2)(Pc)]. This was interpreted as PBM increasing cell metabolism (ATP production) and the consequent higher uptake of the ruthenium phthalocyanine compounds and more efficient apoptosis. The use of metal-based photosensitizers combined with light therapy may represent an advance in the field of photodynamic therapy. (AU)

Processo FAPESP: 16/12707-0 - Citotoxicidade e foto-citotoxicidade de compostos rutênio-ftalocianinas como produtores de óxido nítrico e oxigênio singleto em linhagens de células cancerígenas. Proposta para uma nova classe de metalo-drogas
Beneficiário:Roberto Santana da Silva
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/03746-9 - Efeito sinérgico quimio-fototerapêutico usando complexos rutênio-ftalocianinas associados à cisplatina: avaliação química e fotobiológica em meio aquoso e em sistema de liberação de drogas
Beneficiário:Laísa Bonafim Negri
Linha de fomento: Bolsas no Brasil - Doutorado