Antimicrobial blue light inactivation of international clones of multidrug-resistant Escherichia coli ST10, ST131 and ST648

Autor(es)

dos Anjos, Carolina ^[1] ; Sabino, Caetano P. ^{[2, 3]} ; Bueris, Vanessa ^[4] ; Fernandes, Miriam R. ^[2] ; Pogliani, Fabio C. ^[1] ; Lincopan, Nilton ^{[4, 2]} ; Sellera, Fabio P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP - Brazil

^[3] Sci & Commercial LTD, BioLambda, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 27, p. 51-53, SEP 2019.
• Citações Web of Science: 0

Resumo

Background: International clones of multidrug-resistant Escherichia coli have been a leading cause of human and animal infections worldwide. Microbial inactivation by blue light has been proposed as an effective treatment for superficial infections and surface contamination. Aim: To evaluate the inactivation efficacy of blue light irradiation against high-risk multidrug-resistant strains of E. coli. Methods: Blue LED light (lambda = 410 nm) was used to inactivate in vitro suspensions of colistin- broad-spectrum cephalosporin-, or carbapenem-resistant E. coli strains belonging to sequence types (STs) ST10, ST131 and ST648, carrying mcr-1, bla(CTX-M) or bla(KPC-2) genes. Results: Our results showed that all E. coli strains were susceptible to blue light irradiation, independently of antibiotic resistance and virulence profiles. In addition, blue light irradiation induced a strain-specific and dose-dependent bacterial effect. Conclusion: Our results support use of blue light as a promising antimicrobial option against MDR pathogens, including high-risk clones of E. coli displaying resistance to polymyxins or broad-spectrum beta-lactam antibiotics. (AU)

• Processo FAPESP: 15/13527-2 - Relação ancestral, pan-resistoma plasmidial de Escherichia coli produtora de b-lactamases de espectro estendido (CTX-M) e métodos de cura plasmidial
• Beneficiário: Miriam Rodriguez Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil
• Beneficiário: Nilton Erbet Lincopan Huenuman
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/25095-2 - Fotoinativação bacteriana de patógenos da mastite por meio da luz azul: mecanismos de ação e segurança celular - estudo pré clínico
• Beneficiário: Carolina dos Anjos
• Modalidade de apoio: Bolsas no Brasil - Doutorado