Antimicrobial blue light inactivation of international clones of multidrug-resistant Escherichia coli ST10, ST131 and ST648

Author(s)

dos Anjos, Carolina ^[1] ; Sabino, Caetano P. ^{[2, 3]} ; Bueris, Vanessa ^[4] ; Fernandes, Miriam R. ^[2] ; Pogliani, Fabio C. ^[1] ; Lincopan, Nilton ^{[4, 2]} ; Sellera, Fabio P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo, SP - Brazil

^[3] Sci & Commercial LTD, BioLambda, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 27, p. 51-53, SEP 2019.
• Web of Science Citations: 0

Abstract

Background: International clones of multidrug-resistant Escherichia coli have been a leading cause of human and animal infections worldwide. Microbial inactivation by blue light has been proposed as an effective treatment for superficial infections and surface contamination. Aim: To evaluate the inactivation efficacy of blue light irradiation against high-risk multidrug-resistant strains of E. coli. Methods: Blue LED light (lambda = 410 nm) was used to inactivate in vitro suspensions of colistin- broad-spectrum cephalosporin-, or carbapenem-resistant E. coli strains belonging to sequence types (STs) ST10, ST131 and ST648, carrying mcr-1, bla(CTX-M) or bla(KPC-2) genes. Results: Our results showed that all E. coli strains were susceptible to blue light irradiation, independently of antibiotic resistance and virulence profiles. In addition, blue light irradiation induced a strain-specific and dose-dependent bacterial effect. Conclusion: Our results support use of blue light as a promising antimicrobial option against MDR pathogens, including high-risk clones of E. coli displaying resistance to polymyxins or broad-spectrum beta-lactam antibiotics. (AU)

• FAPESP's process: 15/13527-2 - Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods
• Grantee: Miriam Rodriguez Fernandes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/25095-2 - BACTERIAL PHOTOINACTIVATION OF MASTITIS PATHOGENS BY BLUE LIGHT: MECHANISMS OF ACTION AND CELL SECURITY - PRE-CLINICAL STUDY
• Grantee: Carolina dos Anjos
• Support Opportunities: Scholarships in Brazil - Doctorate