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Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods

Grant number: 15/13527-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2017
Effective date (End): November 04, 2018
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Nilton Erbet Lincopan Huenuman
Grantee:Miriam Rodriguez Fernandes
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):17/16754-5 - Characterization of plasmids associated with the spread of the colistin resistance MCR-1 gene in clinically significant Enterobacteriaceae from South American and European Countries, BE.EP.DR

Abstract

Escherichia coli strains producing ²-lactamase extended spectrum (ES²L) of CTX-M type are endemic in Brazil, prevalent in cases of nosocomial infection and outpatient. Recently, strains producing CTX-M have spread to urban environments and pets and / or production, additionally affecting agribusiness. The versatility and variety of pathogenic or contaminant species as is inherent in its position as commensal microbiota of a wide variety of organisms. Thus, the selection and dissemination of ES²L genotypes within the species, is a predictable genetic event that has been facilitated by the acquisition of blaCTX-M genes, mobilized by plasmids. This study aims to elucidate aspects still unknown on the ancestral relationship and pan-resistoma plasmids that carry genes of the CTX-M type (blaCTX-M-2, blaCTX-M-8 and M-blaCTX-15) strains Escherichia coli isolated from clinical specimens (human and veterinary), the microbiota of humans and companion animals, production animals and urban aquatic environments in Brazil. In addition, plasmid curing methods will be evaluated in vitro and in vivo (invertebrate model Galleria mellonela) in order to propose solutions applicable in clinical practice and agribusiness, to reduce the problem of Plasmid-mediated resistance associated with high rates of morbidity and mortality.

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CARVALHO, MARCELO P. N.; FERNANDES, MIRIAM R.; SELLERA, FABIO P.; LOPES, RALF; MONTE, DANIEL F.; HIPPOLITO, ALICIA G.; MILANELO, LILIANE; RASO, TANIA F.; LINCOPAN, NILTON. International clones of extended-spectrum beta-lactamase (CTX-M)-producing Escherichia coli in peri-urban wild animals, Brazil. TRANSBOUNDARY AND EMERGING DISEASES, APR 2020. Web of Science Citations: 0.
FERNANDES, MIRIAM R.; SELLERA, FABIO P.; MOURA, QUEZIA; ESPOSITO, FERNANDA; SABINO, CAETANO P.; LINCOPAN, NILTON. Identification and genomic features of halotolerant extended-spectrum-beta-lactamase (CTX-M)-producing Escherichia coli in urban-impacted coastal waters, Southeast Brazil. Marine Pollution Bulletin, v. 150, JAN 2020. Web of Science Citations: 0.
DOS ANJOS, CAROLINA; SABINO, CAETANO P.; BUERIS, VANESSA; FERNANDES, MIRIAM R.; POGLIANI, FABIO C.; LINCOPAN, NILTON; SELLERA, FABIO P. Antimicrobial blue light inactivation of international clones of multidrug-resistant Escherichia coli ST10, ST131 and ST648. Photodiagnosis and Photodynamic Therapy, v. 27, p. 51-53, SEP 2019. Web of Science Citations: 0.
GOLDBERG, DAPHNE W.; FERNANDES, MIRIAM R.; SELLERA, FABIO P.; COSTA, DANIELA G. C.; LOUREIRO BRACARENSE, ANA PAULA; LINCOPAN, NILTON. Genetic background of CTX-M-15-producing Enterobacter hormaechei ST114 and Citrobacter freundii ST265 co-infecting a free-living green turtle (Chelonia mydas). ZOONOSES AND PUBLIC HEALTH, v. 66, n. 5, p. 540-545, AUG 2019. Web of Science Citations: 0.
SELLERA, FABIO P.; FERNANDES, MIRIAM R.; SABINO, CAETANO P.; DE FREITAS, LAURA M.; DA SILVA, LUCIANO C. B. A.; POGLIANI, FABIO C.; RIBEIRO, MARTHA S.; HAMBLIN, MICHAEL R.; LINCOPAN, NILTON. Effective treatment and decolonization of a dog infected with carbapenemase (VIM-2)-producing Pseudomonas aeruginosa using probiotic and photodynamic therapies. VETERINARY DERMATOLOGY, v. 30, n. 2, p. 170+, APR 2019. Web of Science Citations: 1.
FERNANDES, MIRIAM R.; SELLERA, FABIO P.; MOURA, QUEZIA; GASPAR, VITOR C.; CERDEIRA, LOUISE; LINCOPAN, NILTON. International high-risk clonal lineages of CTX-M-producing Escherichia coli F-ST648 in free-roaming cats, South America. INFECTION GENETICS AND EVOLUTION, v. 66, p. 48-51, DEC 2018. Web of Science Citations: 2.
SELLERA, FABIO P.; FERNANDES, MIRIAM R.; MOURA, QUEZIA; SOUZA, TIAGO A.; NASCIMENTO, CRISTIANE L.; CERDEIRA, LOUISE; LINCOPAN, NILTON. Draft genome sequence of an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST644 isolated from a footpad infection in a Magellanic penguin (Spheniscus magellanicus). JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 12, p. 88-89, MAR 2018. Web of Science Citations: 1.
ESPOSITO, FERNANDA; FERNANDES, MIRIAM R.; LOPES, RALF; MUNOZ, MARIA; SABINO, CAETANO P.; CUNHA, MARCOS P.; SILVA, KETRIN C.; CAYO, RODRIGO; MARTINS, WILLAMES M. B. S.; MORENO, ANDREA M.; KNOBL, TEREZINHA; GALES, ANA C.; LINCOPAN, NILTON. Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential. Journal of Clinical Microbiology, v. 55, n. 12, p. 3454-3465, DEC 2017. Web of Science Citations: 19.

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