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Molecular characterization of plasmids harboring extended-spectrum Beta-lactamase-encoding genes in Enterobacteriaceas isolated from swine

Grant number: 12/08332-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2012
Effective date (End): December 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal researcher:Andrea Micke Moreno
Grantee:Ketrin Cristina da Silva
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


In Brazil, the antimicrobial resistance researches in animal origin bacterial strains, including swine, are rare or bit depth. In this regard, the molecular characterization of plasmids harboring antimicrobial resistance genes as bacterial strains carrying these plasmids will be very important to epidemiology and will cause a great impact in antimicrobial resistance research. Plasmids are mobile genetic elements frequently associated to emergence and spread of antimicrobial resistance. The aim of this study is to contribute to clarify the Extended- spectrum Beta-lactamases (ESBL) origin and dissemination in Brazilian animals through molecular characterization of plasmids harboring blaESBL genes extracted from Escherichia coli, Salmonella enterica and Klebsiella pneumoniae isolated from commercial swine stool and establish the clonal relationship between the bacterial strains by Multilocus Sequence Typing (MLST). The antimicrobial susceptibility profile to beta-lactam antibiotics will determined by disc-diffusion method (Kirby-Bauer) and confirmed by Minimal Inhibitory Concentration determination in resistant strains.The ESBL production will be identified by double-disc synergy test (DDT) and research of blaCTX-M, blaTEM e blaSHV genes by PCR. The plasmids harboring ESBL encoding genes will be extracted and transformed into Escherichia coli TOP10 recipient strain and characterized according incompatibility group and molecular weight. The profile of strains carrying these plasmids will be determined by MLST and deposited in specific database ( (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, KETRIN C.; MORENO, MARINA; CABRERA, CARLOS; SPIRA, BENY; CERDEIRA, LOUISE; LINCOPAN, NILTON; MORENO, ANDREA M. First Characterization of CTX-M-15-Producing Escherichia coli Strains Belonging to Sequence Type (ST) 410, ST224, and ST1284 from Commercial Swine in South America. Antimicrobial Agents and Chemotherapy, v. 60, n. 4, p. 2505-2508, APR 2016. Web of Science Citations: 8.

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